PMID: 11905997Mar 22, 2002Paper

Nicotine activates the extracellular signal-regulated kinase 1/2 via the alpha7 nicotinic acetylcholine receptor and protein kinase A, in SH-SY5Y cells and hippocampal neurones

Journal of Neurochemistry
Federico Dajas-BailadorSusan Wonnacott

Abstract

Neuronal nicotinic acetylcholine receptors (nAChR) can modulate many cellular mechanisms, such as cell survival and memory processing, which are also influenced by the serine/threonine protein kinases ERK1/2. In SH-SY5Y cells and hippocampal neurones, nicotine (100 microM) increased the activity of ERK1/2. This effect was Ca2+ dependent, and prevented by the alpha7 nAChR antagonist alpha-bungarotoxin (alpha-Bgt) and an inhibitor (PD98059) of the upstream kinase MEK. To determine the intervening steps linking Ca2+ entry to MEK-ERK1/2 activation, inhibitors of Ca2+-dependent kinases were deployed. In SH-SY5Y cells, selective blockers for PKC (Ro 31-8220), CaM kinase II (KN-62) or PI3 kinase (LY 294002) failed to inhibit the nicotine-evoked increase in ERK1/2 activity. In contrast, two structurally different inhibitors of PKA (KT 5720 and H-89) completely prevented the nicotine-dependent increase in ERK1/2 activity. Inhibition of the nicotine-evoked increase in ERK1/2 activity by H-89 was also observed in hippocampal cultures. Down stream of PKA, the activity of B-Raf was significantly decreased by nicotine in SH-SY5Y cells, as determined by direct measurement of MEK1 phosphorylation or in vitro kinase assays, whereas the modulati...Continue Reading

Citations

Nov 19, 2002·Journal of Neurobiology·Edna F R PereiraEdson X Albuquerque
Apr 14, 2010·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Wen-Yan Cui, Ming D Li
Aug 5, 2008·Journal of Molecular Neuroscience : MN·Thomas K CresonGuang Chen
Aug 12, 2008·Molecular Neurobiology·Justin W Kenney, Thomas J Gould
Aug 17, 2012·Neurotoxicity Research·Laura L Hurley, Yousef Tizabi
Mar 17, 2004·Experimental Cell Research·Vu Thuong NguyenSergei A Grando
Aug 29, 2009·Journal of Proteome Research·Joao A PauloEdward Hawrot
May 19, 2009·Acta Pharmacologica Sinica·Jian-xin Shen, Jerrel L Yakel
May 23, 2009·Nature Reviews. Immunology·Kevin J Tracey
Jul 1, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Laura Del BarrioManuela G López
Feb 16, 2007·Neuroreport·Saara NuutinenSusan Wonnacott
Nov 13, 2010·Addiction Biology·Bernd LenzStefan Bleich
Mar 19, 2009·Pharmacological Reviews·Steven D BuckinghamDavid B Sattelle
Aug 20, 2011·American Journal of Respiratory Cell and Molecular Biology·Monika I HollenhorstMartin Fronius
May 1, 2011·Therapeutic Advances in Chronic Disease·Jun KawamataShun Shimohama
Apr 7, 2011·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Norifumi ShiodaKohji Fukunaga
Jan 16, 2007·Molecular Neurobiology·Thomas J Gould
May 1, 2012·Pharmaceutical Patent Analyst·Federica AmigoniPaolo Pevarello
Jun 28, 2014·Neurochemical Research·J Alex Grizzell, Valentina Echeverria
Jan 29, 2014·Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco·Rick E BernardiAnita C Hansson
Jan 13, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Gabriella RodaMarco De Amici
Apr 12, 2012·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Jehan Mohamed AlsharnoubiOla Didrik Saugstad
Mar 10, 2010·Brain Research·Earl J GubbinsJinhe Li
Dec 22, 2015·Physiology & Behavior·Munir Gunes Kutlu, Thomas J Gould
Dec 30, 2008·Neurochemistry International·M Mousavi, E Hellström-Lindahl
Jan 9, 2008·Neuroscience and Biobehavioral Reviews·Concepcion Conejero-GoldbergLuis Ulloa
Oct 2, 2007·Dermatologic Clinics·Adone BaroniVincenzo Ruocco

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.