Nicotine inhibits mineralization of human dental pulp cells

Journal of Endodontics
Manabu YanagitaShinya Murakami

Abstract

Nicotine is a major component of tobacco smoke, and signals via nicotinic acetylcholine receptors (nAChR). However, little is known about the effects of nicotine on human dental pulp cells (HDPCs). In this study, we assessed the effects of nicotine on mineralization in HDPCs. We confirmed messenger RNA expression of nAChR subunits and examined the effects of nicotine on expression of extracellular matrices (ECMs), alkaline phosphatase (ALP) activity, and mineralized nodule formation by HDPCs. Gene expression of nAChR subunits alpha1, alpha2, alpha 4, alpha 5, alpha 6, alpha 7, beta1, beta2, and beta 4 was detected in HDPCs. Interestingly, the messenger RNA expression of dentin matrix acidic phosphoprotein-1, bone sialoprotein, and ALP activity were significantly reduced in nicotine-treated HDPC. In addition, mineralized nodule formation, which was examined by alizarin red staining, was also inhibited in HDPCs by the same treatment. These results indicate that nicotine suppresses the cytodifferentiation and mineralization of HDPCs, possibly via nAChR.

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Citations

Jul 27, 2011·Journal of Endodontics·Lifen LiHelena H Ritchie
Oct 21, 2009·Journal of Endodontics·Yoshio ShimabukuroShinya Murakami
Jul 28, 2015·Scientific Reports·Aya HashimotoEiichi Tamiya
Jul 19, 2015·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Juliana Medeiros de CamposRenato Corrêa Viana Casarin
Apr 15, 2015·Stem Cells and Development·Petra HilkensIvo Lambrichts
Jun 6, 2015·Journal of Dental Research·K MoriS Murakami
Nov 5, 2013·Journal of Dental Research·M YanagitaS Murakami
May 23, 2018·Journal of Public Health·Priyanka HuilgolJ Michael Wells
Feb 22, 2011·Journal of Agricultural and Food Chemistry·Christina L RaineyJohn V Goodpaster

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