Nicotine-responsive genes in cultured embryonic mouse lung buds: interaction of nicotine and superoxide dismutase

Pharmacological Research : the Official Journal of the Italian Pharmacological Society
Carol WuenschellPaul Marjoram

Abstract

Nicotine exposure during prenatal development may be a cause of the abnormal lung function seen in infants born to smoking women. Previously we used an organ culture system to demonstrate that nicotine directly affects branching morphogenesis and gene expression in embryonic mouse lung buds. Here we attempt to identify genes potentially involved in the nicotine response and explore the relationship between gene expression changes and stimulation of branching. DNA microarray technology, analyzed by DChip software, and semi-quantitative RT-PCR were applied to RNA samples from embryonic lung buds grown in presence or absence of nicotine. Four genes, BAX, calcyclin, osteopontin and Cu-Zn superoxide dismutase (SOD1), identified by the microarray as showing changes in mRNA level with nicotine treatment were investigated in detail. RT-PCR showed that nicotine exposure resulted in significant decreases in mRNA levels for BAX, calcyclin and osteopontin, but nicotine did not affect the mRNA level of SOD1. Nicotine-induced changes in BAX, calcyclin and osteopontin mRNAs showed a general correlation with stimulation of branching, implying a common mechanism for effects of nicotine on branching and on gene expression. BAX, calcyclin and ost...Continue Reading

Citations

Sep 12, 2007·Apoptosis : an International Journal on Programmed Cell Death·Reinhard ZeidlerStephan Lang
Dec 4, 2004·American Journal of Respiratory Cell and Molecular Biology·Edwin C JesudasonPaul D Losty
Dec 1, 2007·International Journal of Chronic Obstructive Pulmonary Disease·Becky A Mercer, Jeanine M D'Armiento
Apr 12, 2005·Journal of Cardiovascular Pharmacology·Salma Toma HannaRui Wang
Jun 29, 2006·Journal of Psychosocial Oncology·Les Gallo-Silver, Michael O Weiner

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