Nicotinic acetylcholine receptor channels are influenced by the physical state of their membrane environment

Biophysical Journal
L P ZanelloF J Barrantes

Abstract

We investigated the effect of the physical state of the cell membrane on the activity of the nicotinic acetylcholine receptor (AChR) in various clonal cell lines transfected with the cDNAs of embryonic or adult AChR by measuring single-channel properties and some membrane physicochemical properties as a function of temperature. Unitary conductance and channel closing rate, alpha, had Q(10) values of 1.2 and 2.2, respectively. Using Eyring's transition state theory, it was calculated that both embryonic and adult-type AChR had relatively low thermal sensitivity of ionic conductance and activation energy (E(a) of 3.0-5.0 kcal-mol(-1) at 20 degrees C), indicating that once the AChR channel opens, ion movement is dominated by diffusional processes. Channel closure exhibited higher energy requirements, with E(a) values of about 13 kcal-mol(-1). This process appears to be more endothermic (higher delta H(a) values) than ion permeation, and it is plausible that the energy acquired by the system can be used in the maintenance of its degree of order, as revealed by the delta S(a) 0 calculated for channel closure. The influence of the membrane environment on AChR function is reinforced by the observation that the conductance of the same,...Continue Reading

Citations

Feb 14, 2002·Chemistry and Physics of Lipids·Sathyamangalam V BalasubramanianRobert M Straubinger
Nov 27, 2002·European Journal of Pharmacology·Kenzo KudoTakeshi Kashimoto
Aug 1, 1997·Current Opinion in Structural Biology·O G Mouritsen, K Jørgensen
Jan 12, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·M T Lima-Landman, A J Lapa
Apr 24, 1999·The European Journal of Neuroscience·A M RoccamoF J Barrantes
Feb 16, 2011·Biophysical Journal·Shaweta Gupta, Anthony Auerbach
Dec 8, 2007·The Journal of Physiology·Deeptankar Demazumder, James P Dilger
Apr 4, 2015·Biochimica Et Biophysica Acta·Francisco J Barrantes
Oct 8, 2013·Biophysical Journal·Constanza B KamerbeekFrancisco J Barrantes
Jul 27, 2005·Biochimica Et Biophysica Acta·Soumi Mukherjee, Amitabha Chattopadhyay
Dec 2, 2004·Brain Research. Brain Research Reviews·F J Barrantes
Dec 6, 2007·Journal of Neurochemistry·Francisco J Barrantes
May 31, 2008·The Journal of Biological Chemistry·Gaspar A Fernández NievasSilvia S Antollini
Jan 28, 1999·The Journal of Biological Chemistry·C G Bevans, A L Harris
Jun 29, 2017·European Biophysics Journal : EBJ·Florian P M Kohn, Ramona Ritzmann
Nov 21, 2021·The FEBS Journal·Diego J B Orts, Manoel Arcisio-Miranda

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.