Nicotinic actions on neurones of the central autonomic area in rat spinal cord slices

The Journal of Physiology
A BordeyJérôme Trouslard

Abstract

1. Nicotinic responses and actions on excitatory synaptic activity were studied in eighty-four neurones in the region dorsal to the central canal (lamina X) in transverse thoracolumbar spinal cord slices of neonate (P2-P10) rats by using the whole-cell patch-clamp technique. 2. Neurones (n = 15) labelled with Lucifer Yellow, showed the typical morphology of sympathetic preganglionic neurones (SPNs) in the central autonomic area (CA). Unlabelled neurones of comparable morphology were visually identified and recorded. 3. All neurones recorded responded to the nicotinic acetylcholine receptor (nAChR) agonist, DMPP. Under current-clamp conditions, pressure ejections of DMPP depolarized cells and induced the discharge of action potentials. Tetrodotoxin suppressed action potentials but not DMPP-induced depolarization. 4. Under voltage-clamp conditions at a holding potential (Vh) of -50 mV, DMPP induced a transient inward current (which reversed around 0 mV) and an increase in membrane current noise in 50% of the recorded neurones. In the others, DMPP increased membrane current noise without measurable inward current. The current-voltage relationship showed strong inward rectification at holding potentials more positive than 0 mV. 5. ...Continue Reading

Citations

Sep 30, 2009·Brain Research·Andrea H GaedePaul M Pilowsky
Apr 15, 1999·Annual Review of Neuroscience·A B MacDermottS A Siegelbaum
May 16, 2002·Clinical and Experimental Pharmacology & Physiology·Ida J Llewellyn-Smith
Jan 29, 2002·Journal of Neuroscience Research·Sergio FucileFabrizio Eusebi
Jun 14, 2008·Journal of Neurophysiology·Katharina A Quinlan, James T Buchanan
Apr 16, 2019·Pain·Volodymyr KrotovNana Voitenko
Nov 23, 2017·Frontiers in Cellular Neuroscience·Volodymyr KrotovNana Voitenko
Dec 22, 1999·Journal of Neurophysiology·A Bordey, H Sontheimer
Jan 22, 1998·Journal of Medicinal Chemistry·M W HolladayJ K Lynch

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