Nicotinic cholinergic stimulation promotes survival and reduces motility of cultured rat cerebellar granule cells

Neuroscience
Sergio FucileFabrizio Eusebi

Abstract

Despite many studies on the functional expression of neuronal nicotinic acetylcholine receptors (nAChRs), an exhaustive description of the long-term effects of nicotine (Nic) stimulation in cerebellar granules is still far to be completed. For this reason, we addressed the experiments stimulating cultured cerebellar granule neurons (CGN) with Nic, focusing on the effects on cell motility and survival. Using electrophysiological and Ca(2+)-fluorescence techniques, we found a subset of rat CGN that responded to Nic by inward whole cell currents and by short-delay Ca(2+) transients. These responses were mediated through both homomeric and heteromeric nAChRs, as assessed by their sensitivity to alpha-bungarotoxin (alpha-BTX), dihydro-beta-erythroidine (DHbetaE), methyllicaconitine (MLA) and 5-hydroxyindole (5OH-indole). Once established the expression of alpha-BTX-sensitive and insensitive nAChRs and their ability to trigger Ca(2+) responses in CGN, we aimed at investigating their possible role on cell survival and motility. We demonstrate that Nic stimulation significantly increases the survival of CGN exposed to the apoptosis-promoting low K(+) medium. This anti-apoptotic effect is likely mediated through alpha7* nAChRs since we ...Continue Reading

References

May 1, 1995·Brain Research. Molecular Brain Research·M DidierS Bursztajn
Apr 2, 1993·Science·H Komuro, P Rakic
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S R D'MelloP Calissano
Dec 21, 1995·Brain Research. Developmental Brain Research·J A CourtF Clementi
Jun 1, 1996·Current Opinion in Neurobiology·D S McGehee, L W Role
Mar 28, 1998·The European Journal of Neuroscience·M AlkondonE X Albuquerque
Aug 7, 1998·Brain Research. Developmental Brain Research·E Hellström-LindahlA Nordberg
Apr 15, 1999·Annual Review of Neuroscience·A B MacDermottS A Siegelbaum
Jan 3, 2001·The European Journal of Neuroscience·L A OpanashukK F Hauser
Dec 26, 2001·The Journal of Biological Chemistry·Erin L MeyerScott W Rogers
Jun 22, 2002·Brain : a Journal of Neurology·M LeeE Perry
Sep 5, 2002·Nature Reviews. Neuroscience·David F Owens, Arnold R Kriegstein
Dec 13, 2003·Cell Calcium·Sergio Fucile

❮ Previous
Next ❯

Citations

Sep 12, 2007·Apoptosis : an International Journal on Programmed Cell Death·Reinhard ZeidlerStephan Lang
Feb 8, 2006·Biogerontology·J Krøll
Mar 12, 2005·The Journal of Physiology·Sergio FucileFabrizio Eusebi
Mar 10, 2009·Pharmacology & Therapeutics·Jennifer B DwyerFrances M Leslie
Jun 30, 2009·Seminars in Cell & Developmental Biology·Huibert D MansvelderLorna W Role
Aug 12, 2005·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Jennifer L ZechelW David Lust
Jul 30, 2005·Journal of Cellular Biochemistry·Matthew D ThullberyKathleen M George
Feb 27, 2015·Brain Structure & Function·Natalia OmelchenkoSusan R Sesack
Mar 6, 2007·Virchows Archiv : an International Journal of Pathology·Anna Maria LavezziLuigi Matturri
Aug 26, 2016·Reviews in the Neurosciences·Changzheng ZhangTifei Yuan
Nov 20, 2015·Journal of Neurophysiology·Kigen James CurticeRussell W Teichert

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis