Niemann-Pick C2 Proteins: A New Function for an Old Family

Frontiers in Physiology
Jiao ZhuGuirong Wang

Abstract

Niemann-Pick proteins type C2 (NPC2) are carriers of cholesterol in vertebrates, with a single member in each species. The high sequence conservation between mammals and across vertebrates is related to their common function. In contrast, NPC2 proteins in arthropods have undergone extensive duplication and differentiation, probably under environmental pressure, and are likely to have different functions. Recent studies have suggested that in arthropods these proteins might act as carriers for semiochemicals and other hydrophobic compounds. In this study we focused on the function of a specific NPC2 gene in the mothHelicoverpa armigera(HarmNPC2-1). This protein binds several flavonoids with micromolar dissociation constants. The best ligand was gossypol, present in cotton, one of the main host plants forH. armigera. Western blot revealed the presence of HarmNPC2-1 in different parts of the body, including the antennae, proboscis, and abdomen. In the antennae,in situhybridization experiments produced strong staining in auxiliary cells at the base of sensilla trichodea, basiconica, coeloconica, and chaetica. Immunocytochemistry confirmed the expression of the protein in sensilla chaetica. Our results support a role of semiochemica...Continue Reading

References

Jan 1, 1982·The Biochemical Journal·P PelosiA M Pisanelli
Jan 1, 1994·Critical Reviews in Biochemistry and Molecular Biology·P Pelosi
Apr 18, 1998·European Journal of Biochemistry·S MarcheseP Pelosi
Nov 4, 2000·Biochimica Et Biophysica Acta·A Cavaggioni, C Mucignat-Caretta
Nov 4, 2000·Biochimica Et Biophysica Acta·M TegoniC Cambillau
Jun 11, 2002·Biochemical and Biophysical Research Communications·Liping BanPaolo Pelosi
Sep 25, 2002·The European Journal of Neuroscience·Jürgen KriegerHeinz Breer
Feb 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Natalia FriedlandAnn M Stock
Oct 3, 2003·Cellular and Molecular Life Sciences : CMLS·M CalvelloP Pelosi
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Nov 23, 2005·Bioinformatics·Konstantin ArnoldTorsten Schwede
Jun 19, 2007·The Journal of Biological Chemistry·Sujuan XuAnn M Stock
Sep 10, 1981·Nature·R G Vogt, L M Riddiford
Oct 22, 2008·Nucleic Acids Research·Florian KieferTorsten Schwede
Feb 24, 2009·Biochimica Et Biophysica Acta·Judith Storch, Zhi Xu
Jun 1, 2011·Nucleic Acids Research·Aurélien GrosdidierOlivier Michielin
Jul 23, 2013·Biochemical and Biophysical Research Communications·Liping BanPaolo Pelosi
Feb 26, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yuko IshidaToshimasa Yamazaki
Aug 12, 2014·Biochemical Society Transactions·Robert J BeynonJane L Hurst
Sep 16, 2014·Frontiers in Physiology·Paolo PelosiFrancesca R Dani
Jan 25, 2016·Science China. Life Sciences·Xiu TianChangqing Yang
May 1, 2016·Biochemical and Biophysical Research Communications·Jiao ZhuPaolo Pelosi
Aug 24, 2016·Proceedings of the National Academy of Sciences of the United States of America·Xiaochun LiSuzanne R Pfeffer
Oct 18, 2016·Insect Biochemistry and Molecular Biology·Corinna KremplNicole Joußen
Oct 18, 2016·Insect Biochemistry and Molecular Biology·Immacolata IovinellaFrancesca Romana Dani
May 10, 2017·Biological Reviews of the Cambridge Philosophical Society·Paolo PelosiFrancesca R Dani

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Citations

Jul 29, 2018·Applied Microbiology and Biotechnology·Paolo PelosiWolfgang Knoll
Sep 29, 2018·Sensors·Paolo PelosiWolfgang Knoll
Jul 6, 2018·Frontiers in Physiology·Immacolata IovinellaFrancesca R Dani
Oct 7, 2020·Open Biology·Hayden R Schmidt, Richard Benton
Jan 23, 2019·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Chunli XiuYongjun Zhang
May 1, 2021·Genome Biology and Evolution·Jiao ZhuPaolo Pelosi
Jul 3, 2021·International Journal of Molecular Sciences·Jiao ZhuPaolo Pelosi
Mar 15, 2019·Journal of Agricultural and Food Chemistry·Bowen TangChunni Zhang
Sep 5, 2021·Biological Reviews of the Cambridge Philosophical Society·Paolo Pelosi, Wolfgang Knoll
Sep 30, 2019·International Archives of Allergy and Immunology·Eduardo Santos da SilvaNeuza Maria Alcantara-Neves

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Methods Mentioned

BETA
PCR
transmission electron microscopy
glycosylation

Software Mentioned

Chimera
SWISS MODEL
SWISS DOCK
cellSens
SwissModel

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