NifA-dependent expression of glutamate dehydrogenase in Rhizobium etli modifies nitrogen partitioning during symbiosis

Molecular Plant-microbe Interactions : MPMI
A MendozaJ Mora

Abstract

Constitutive expression of foreign glutamate dehydrogenase in Rhizobium etli inhibits bean plant nodulation (A. Mendoza, A. Leija, E. Martínez-Romero, G. Hernández, and J. Mora. Mol. Plant-Microbe Interact. 8:584-592, 1995). Here we report that this inhibition is overcome when controlling gdhA expression by NifA, thus delaying the GDH activity onset after nodule establishment. Expression of gdhA modifies the nitrogen partitioning inside the bacteroid, where newly synthesized ammonia is preferentially incorporated into the amino acid pool instead of being exported to the infected cells. As a consequence, the fixed nitrogen transport to the leaves, measured as the ureides content in xylem sap, is significantly reduced. Nitrogenase activity, although not nifHDK expression, is significantly reduced in bacteroids expressing gdhA, probably due to the utilization of energy and reducing power for nitrogen assimilation. Here we show that ammonia assimilation inside R. etli bacteroids is active, albeit at low levels, and when enhanced is deleterious to the symbiotic performance. This leads us to believe that further reduction of the basal nitrogen metabolism in the bacteroid might stimulate the nitrogenase activity and increase the nitro...Continue Reading

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Citations

Jul 15, 2009·Proceedings of the National Academy of Sciences of the United States of America·J PrellP S Poole
Jul 5, 2001·Molecular Plant-microbe Interactions : MPMI·R TatèE J Patriarca
Jun 1, 2002·Microbiology and Molecular Biology Reviews : MMBR·Eduardo J PatriarcaMaurizio Iaccarino
Oct 24, 2008·Phytopathology·Mojibur R KhanFiona M Doohan

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