Nigella sativa L. seed extract modulates the neurotransmitter amino acids release in cultured neurons in vitro.

Journal of Biomedicine & Biotechnology
Tarek El-NaggarMaría Emilia Carretero

Abstract

Nigella sativa L. (NS) has been used for medicinal purposes since ancient times. This study aimed to investigate the cytotoxicity of NS dry methanolic extract on cultured cortical neurons and its influence on neurotransmitter release, as well as the presence of excitatory (glutamate and aspartate) and inhibitory amino acids (gamma-aminobutyric acid-GABA-and glycine) in NS extract. Cultured rat cortical neurons were exposed to different times and concentrations of NS dry methanolic extract and cell viability was then determined by a quantitative colorimetric method. NS did not induce any toxicity. The secretion of different amino acids was studied in primary cultured cortical neurons by high-performance liquid chromatography (HPLC) using a derivation before injection with dansyl chloride. NS modulated amino acid release in cultured neurons; GABA was significantly increased whereas secretion of glutamate, aspartate, and glycine were decreased. The in vitro findings support the hypothesis that the sedative and depressive effects of NS observed in vivo could be based on changes of inhibitory/excitatory amino acids levels.

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Citations

Jan 15, 2014·Journal of Ethnopharmacology·Muhammad Shahdaat Bin SayeedMd Asaduzzaman
Apr 27, 2016·Journal of Traditional and Complementary Medicine·Farimah BeheshtiMohammad Soukhtanloo
Nov 5, 2013·Journal of Ethnopharmacology·T A F EissaM P Gómez-Serranillos
Sep 19, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Sewara J MohammedHeshu S Rahman

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Datasets Mentioned

BETA
MAF
161043

Methods Mentioned

BETA
sedation
flow cytometry
ELISA

Software Mentioned

CYTED

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