PMID: 2783Dec 4, 1975

Nigericin-induced charge transfer across membranes

The Journal of Membrane Biology
V S MarkinL S Jaguzhinsky

Abstract

The electric properties of the bilayer lecithin membranes have been studied in the presence of the antibiotic nigericin. When the antibiotic concentration is about 10(-7) ohm-1 cm-2. The potassium ion concentration gradient gives rise to a transmembrane potential of the order of 40 mV per 10-fold concentration gradient with the side of the higher potassium concentration negative. The transmembrane potential produced by the hydrogen ion concentration gradient is a function of the potassium ion concentration which is equal on both sides of the membrane. For low potassium ion concentrations the hydrogen potential has the expected polarity with the solution having higher concentration of protons negative. For potassium ion concentrations exceeding 0.03 M the hydrogen potential has the reverse polarity. This unexpected result cannot be accounted for in terms of the available simple hypotheses about the charge transport mechanism for nigericin in BLM. In order to account for the experimental results obtained, a theoretical approach has been developed based on the assumption that charge is transported across the membrane by nigericin dimers. The theoretical predicitons are in satisfactory agreement with the experimental results. The m...Continue Reading

References

Nov 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·B C PressmanJ H Johnson
Dec 3, 1971·Biochemical and Biophysical Research Communications·L K SteinraufM Pinkerton
Aug 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·S N GravenH A Lardy
Mar 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·N ShavitA San Pietro
Sep 17, 1968·Biochimica Et Biophysica Acta·E A Liberman, V P Topaly
Sep 27, 1967·Biochemical and Biophysical Research Communications·L Packer
Dec 2, 1967·Nature·E J Harris, B C Pressman
Dec 1, 1972·The Journal of Membrane Biology·S McLaughlin

Citations

Feb 1, 1992·Journal of Bioenergetics and Biomembranes·R AlvaS Estrada
Jan 1, 1987·The Journal of Membrane Biology·M ToroS Estrada
Apr 22, 1977·The Journal of Membrane Biology·M LewinG Sachs
Apr 3, 2002·Drug Development and Industrial Pharmacy·Leo GriniusAlan W Curnow
Jul 10, 2007·International Journal for Parasitology·Ingrid A Fruth, Gustavo Arrizabalaga
Jul 8, 1980·Biochimica Et Biophysica Acta·J Warncke, C L Slayman
Apr 5, 2000·The Journal of Biological Chemistry·F B Schapiro, S Grinstein

Related Concepts

Bacteriocidal Agents
Electric Conductivity
Hydrogen-Ion Concentration
Mathematics
Resting Potentials
Membranes, Artificial
Pandavir
Phosphatidylcholines
Slow-K

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