Nile red-adsorbed gold nanoparticles for selective determination of thiols based on energy transfer and aggregation

Analytical Chemistry
Shih-Ju Chen, Huan-Tsung Chang

Abstract

For the first time, an aqueous solution of 32-nm gold nanoparticles (GNPs), to which Nile red (NR) has been noncovalently adsorbed, has been used for sensing thiols. The as-prepared NRGNPs fluoresce weakly as a result of fluorescence resonance energy transfer between NR and the GNPs. The fluorescence of a solution containing NRGNPs at pH 4.0 increases upon the addition of thiols, but not when amines, acids, alcohols, bovine serum albumin, or hemoglobin are added. This phenomenon allows for the selective determination of thiols such as cysteamine and homocysteine, which have limits of detection of 10.2 and 10.9 nM, respectively, at a signal-to-noise ratio of 3. Interestingly, we have found that the excitation (lambda(ex) = 480 nm), emission (lambda(em) = 610 nm), and mass spectra (m/z 282) of the substance that desorbs from the GNPs in the presence of thiols are different from those of NR (lambda(ex) = 580 nm; lambda(em) = 652 nm; m/z 318), which indicates that a new product forms. When simultaneously conducting fluorescence and colorimetric assays, the selectivity of this approach further improves because at pH 4.0, the color of the NRGNPs does not change in the presence of negatively charged thiols, (e.g., N-(2-mercaptopropion...Continue Reading

References

Mar 24, 1998·Annual Review of Medicine·H RefsumS E Vollset
Sep 25, 1998·Science·M BruchezA P Alivisatos
May 16, 2000·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·N Ghoneim
Apr 3, 2001·Nature Biotechnology·B DubertretA J Libchaber
Jun 14, 2001·Analytical Chemistry·K J AlbertT C Pearce
May 30, 2002·Analytical Chemistry·Jens M KümerIngo Klimant
Aug 9, 2002·Journal of the American Chemical Society·Dustin J MaxwellShuming Nie
Nov 25, 2003·Analytical Chemistry·Keith J Albert, David R Walt
Jan 15, 2004·Journal of the American Chemical Society·Oleksandr RusinRobert M Strongin
Oct 31, 2008·Talanta·Olga NekrassovaRichard G Compton

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Citations

Mar 30, 2013·Journal of the American Society for Mass Spectrometry·Wen-Tsen ChenHuan-Tsung Chang
Jul 23, 2013·Advances in Colloid and Interface Science·Mohammed S Khan Siddaramaiah H
Feb 3, 2012·Chemical Reviews·Krishnendu SahaVincent M Rotello
Nov 10, 2005·Journal of the American Chemical Society·Weihua WangRobert M Strongin
Nov 26, 2009·Journal of the American Chemical Society·Yu-Fen HuangWeihong Tan
Jun 18, 2009·Chemical Communications : Chem Comm·Zonghai ShengHuanchun Chen
Apr 14, 2010·Chemical Communications : Chem Comm·Jiaofang LiuLinyong Zhu
Jun 25, 2010·Chemical Communications : Chem Comm·Cheng-Tai Chen, Yu-Chie Chen
Nov 30, 2011·Bioanalysis·Guillermo Lasarte-AragonésMiguel Valcárcel
Jan 11, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kouki OkuboNobuo Uehara
Nov 17, 2009·Advanced Drug Delivery Reviews·Sarit S AgastiVincent M Rotello
Aug 22, 2015·Chemical Reviews·Xuan YangYounan Xia
Aug 19, 2015·ACS Applied Materials & Interfaces·Joe Gerald Jesu RajMohammed Zourob
Nov 28, 2013·Toxicology Mechanisms and Methods·Diego MateoAna I Haza
Dec 13, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nobuo Uehara, Osamu Yoshida
Jul 14, 2016·Chemistry : a European Journal·Vincent Martinez, Maged Henary
May 16, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yanbing Zu
Jan 15, 2008·Biosensors & Bioelectronics·Bikash Kumar Jena, C Retna Raj
Nov 21, 2014·Chemical Communications : Chem Comm·Maik SchlesingerMark J MacLachlan
Dec 11, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Hani Nasser Abdelhamid, Hui-Fen Wu
Jan 17, 2017·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Hongyu MiGuodong Feng
Oct 18, 2006·Rapid Communications in Mass Spectrometry : RCM·Chih-Lin Su, Wei-Lung Tseng
Oct 2, 2007·Angewandte Chemie·Wei JiangWei Wang
Oct 31, 2007·Chemistry : a European Journal·Bo TangGuiwen Yang
May 15, 2009·Physical Chemistry Chemical Physics : PCCP·Sujit Kumar Ghosh, Tarasankar Pal
Aug 26, 2009·Rapid Communications in Mass Spectrometry : RCM·Ni-Chen ChiangHuan-Tsung Chang

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