Nimesulide inhibits the growth of human esophageal carcinoma cells by inactivating the JAK2/STAT3 pathway

Pathology, Research and Practice
Jun-Ru LiuYue-Min Nan

Abstract

Although selective COX-2 inhibitors have cancer-preventive effects and induce apoptosis, the mechanisms underlying these effects are not fully understood. This study investigated the effects of nimesulide, a selective COX-2 inhibitor, on apoptosis and on the JAK/STAT signaling pathway in Eca-109 human esophageal squamous carcinoma cells. The effects and mechanisms of nimesulide on Eca-109 cell growth were studied in culture and in nude mice with Eca-109 xenografts. Cells were cultured with or without nimesulide and/or the JAK2 inhibitor AG490. Cell proliferation was evaluated using the MTT assay, and apoptosis was investigated. COX-2 mRNA expression was measured using reverse transcription polymerase chain reaction, and protein expression was detected by Western blot analysis, immunohistochemistry, and flow cytometry. Nimesulide significantly inhibited Eca-109 cell viability in vitro in a dose- and time-dependent manner (P<0.05). Nimesulide also induced apoptosis, which was accompanied by a significant decrease in the expression of COX-2 and survivin and an increase in caspase-3 expression. Nimesulide downregulated the phosphorylation levels of JAK2 and STAT3, and JAK2 inhibition by AG490 significantly augmented both nimesulide...Continue Reading

References

Sep 1, 1992·Cancer Metastasis Reviews·J A Hickman
Apr 1, 1993·Endocrine Reviews·R A Schwartzman, J A Cidlowski
Jan 23, 1999·Oncogene·E C LaCasseA E MacKenzie
Apr 20, 2001·Seminars in Hematology·Z DarzynkiewiczP Smolewski
Jan 17, 2002·The Journal of Pathology·Bastiaan P van ReesG Johan A Offerhaus
Jun 1, 2002·Science·David S Aaronson, Curt M Horvath
May 10, 2003·Journal of Molecular and Cellular Cardiology·Yu-Ting XuanRoberto Bolli
Dec 6, 2003·The New England Journal of Medicine·Peter C Enzinger, Robert J Mayer
Jan 15, 2005·Annals of the New York Academy of Sciences·Daniela FoderàMelchiorre Cervello
Jul 23, 2005·Neoplasia : an International Journal for Oncology Research·Florian M CorvinusKarlheinz Friedrich
Jun 15, 2007·International Journal of Oral and Maxillofacial Surgery·M A ScheperJ J Sauk
Jun 19, 2007·Apoptosis : an International Journal on Programmed Cell Death·Allison M HunterRobert G Korneluk
Jun 26, 2008·The Tohoku Journal of Experimental Medicine·Xuan-Fu XuChuan-Yong Guo
Jun 12, 2010·Expert Opinion on Therapeutic Targets·Antonella MigliettaClaudia Bocca
Jun 28, 2012·Molecular BioSystems·Giulia Elisa CambiPietro Amedeo Modesti
May 1, 2013·Journal of Epidemiology·Yingsong LinHideo Tanaka

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Cancer Epigenetics and Chromatin (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on chromatin and its role in cancer epigenetics please follow this feed to learn more.

Cancer Epigenetics Chromatin Complexes (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on chromatin complexes and their role in cancer epigenetics.