Ninjurin1 deficiency attenuates susceptibility of experimental autoimmune encephalomyelitis in mice.

The Journal of Biological Chemistry
Bum Ju AhnKyu-Won Kim

Abstract

Ninjurin1 is a homotypic adhesion molecule that contributes to leukocyte trafficking in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. However, in vivo gene deficiency animal studies have not yet been done. Here, we constructed Ninjurin1 knock-out (KO) mice and investigated the role of Ninjurin1 on leukocyte trafficking under inflammation conditions such as EAE and endotoxin-induced uveitis. Ninjurin1 KO mice attenuated EAE susceptibility by reducing leukocyte recruitment into the injury regions of the spinal cord and showed less adhesion of leukocytes on inflamed retinal vessels in endotoxin-induced uveitis mice. Moreover, the administration of a custom-made antibody (Ab26-37) targeting the Ninjurin1 binding domain ameliorated the EAE symptoms, showing the contribution of its adhesion activity to leukocyte trafficking. In addition, we addressed the transendothelial migration (TEM) activity of bone marrow-derived macrophages and Raw264.7 cells according to the expression level of Ninjurin1. TEM activity was decreased in Ninjurin1 KO bone marrow-derived macrophages and siNinj1 Raw264.7 cells. Consistent with this, GFP-tagged mNinj1-overexpressing Raw264.7 cells increased their TEM activit...Continue Reading

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Citations

Apr 11, 2015·American Journal of Respiratory Cell and Molecular Biology·Carla JenneweinKai Zacharowski
May 25, 2015·Nanomedicine : Nanotechnology, Biology, and Medicine·Su Jung HwangYoumie Park
Nov 7, 2016·Molecular Neurobiology·Hoang LeKyu-Won Kim
Jan 10, 2017·Journal of Cellular Biochemistry·Sung-Jin BaeKyu-Won Kim
Oct 27, 2017·Proceedings of the National Academy of Sciences of the United States of America·Hee Jung YangXinbin Chen
May 16, 2019·PloS One·Melanie KnyJens Fielitz
Dec 18, 2015·International Journal of Oncology·Min Wook ShinKyu-Won Kim
May 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hoon ChoiKyu-Won Kim
Jan 1, 2016·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ruth LyckBritta Engelhardt
Jan 23, 2020·International Journal of Molecular Sciences·Hyun Jin JungSeung Hyun Oh
Oct 26, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Akiho MinoshimaJun-Ichi Kawabe
Feb 1, 2019·Experimental & Molecular Medicine·Sung-Jin BaeKyu-Won Kim
Dec 16, 2020·Neural Regeneration Research·Poornima D E Weerasinghe-MudiyanselageMeejung Ahn
Nov 26, 2021·Science·Kim NewtonNobuhiko Kayagaki

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