NiO Nanoparticles for Exceptionally Stable DNA Adsorption and Its Extraction from Biological Fluids

Langmuir : the ACS Journal of Surfaces and Colloids
Lei ChenJuewen Liu

Abstract

Selective extraction of a small amount of nucleic acids from complex biological samples containing a high concentration of proteins is critical for bioanalytical chemistry. A number of previously published studies have focused on long, double-stranded DNA such as plasmid DNA. On the other hand, we are interested in short oligonucleotides. Nucleic acids have a negatively charged phosphate backbone that interacts with metal oxides strongly, and this may be used to distinguish them from proteins. In this work, a few metal oxide nanoparticles were screened, including NiO, CoO, ZnO, TiO2, CeO2, and Fe3O4 for DNA recovery. NiO had the highest DNA adsorption efficiency from mixtures containing bovine serum albumin or human blood serum. The adsorption of DNA by NiO was further characterized as a function of the pH, salt concentration, DNA length, and DNA sequence. The adsorption mechanism was studied by adding competing chemicals or denaturing agents. A striking observation was the extremely high adsorption affinity of NiO, much higher than that of the other tested oxides. Polyphosphate was the most effective agent for displacing adsorbed DNA, whereas simple inorganic phosphate was less effective. NiO was able to concentrate DNA from a...Continue Reading

References

Oct 6, 2000·Journal of Microbiological Methods·G GiraffaE Neviani
Apr 30, 2005·Biomaterials·Naomi NishimuraHiroyuki Ohno
Nov 30, 2005·Nucleic Acids Research·Min LiHsiao-Sheng Liu
Aug 30, 2008·Analytical Chemistry·Jian WenJames P Landers
Sep 25, 2008·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Neenu VargheseC N R Rao
Jun 23, 2010·Journal of the American Chemical Society·Ying WangYuehe Lin
Mar 15, 2011·Trends in Biotechnology·Ying WangYuehe Lin
Jul 4, 2012·Human Genetics·Andreas ZieglerAnika Grosshennig
Jul 19, 2013·ACS Applied Materials & Interfaces·Rachel PautlerJuewen Liu
Nov 12, 2013·ACS Applied Materials & Interfaces·Wenchao YangZhifeng Shao
Jan 7, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Xu ZhangJuewen Liu
Jun 24, 2014·Chemical Communications : Chem Comm·Biwu Liu, Juewen Liu
Oct 21, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Biwu LiuJuewen Liu
Dec 19, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Biwu Liu, Juewen Liu
Jan 13, 2015·Journal of the American Chemical Society·Biwu LiuJuewen Liu
Feb 4, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Baomei ZhangGuangxi Zhai
Aug 30, 2017·Analytical Chemistry·Lin QiHao Pei
Jun 12, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Xiuzhong WangJuewen Liu

❮ Previous
Next ❯

Citations

Oct 23, 2018·Chemical Communications : Chem Comm·Dai LiJuewen Liu
Oct 3, 2019·Chemical Communications : Chem Comm·Fang XuJuewen Liu
Jul 27, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Mohamad Zandieh, Juewen Liu
Mar 10, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Mohamad Zandieh, Juewen Liu
Dec 6, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Xiuzhong WangJuewen Liu
Nov 5, 2021·Analytical Chemistry·Tianbin XuHongzhou Gu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Langmuir : the ACS Journal of Surfaces and Colloids
Xu ZhangJuewen Liu
Langmuir : the ACS Journal of Surfaces and Colloids
Biwu Liu, Juewen Liu
Langmuir : the ACS Journal of Surfaces and Colloids
Xiuzhong WangJuewen Liu
© 2022 Meta ULC. All rights reserved