Nisin A and Polymyxin B as Synergistic Inhibitors of Gram-positive and Gram-negative Bacteria

Probiotics and Antimicrobial Proteins
Karim NaghmouchiRon Teather

Abstract

Nisin A and polymyxin B were tested alone and in combination in order to test their antagonism against Listeria innocua HPB13 and Escherichia coli RR1, respectively. While the combination of both antibacterial substances was synergistically active against both target bacteria, nisin A alone did not show any inhibition of E. coli RR1. The nisin A/polymyxin B combination at 1.56/2.5 μg ml(-1) caused lysis of about 35.86 ± 0.35 and 73.36 ± 0.14% of L. innocua HPB13 cells after 3 and 18 h, respectively. Polymyxin B at 0.12 μg ml(-1) and nisin A/polymyxin B at 4.64/0.12 μg ml(-1) decreased the numbers of viable E. coli RR1 cells by about 0.23 and 0.65 log10 CFU ml(-1), respectively, compared to the control. Our data suggest that the concentration of nisin A required for the effective control of pathogenic strains Listeria spp. could be lowered considerably by combination with polymyxin B. The use of lower concentrations of nisin A or polymyxin B should slow the emergence of bacterial populations resistant to these agents.

References

Nov 1, 1991·European Journal of Biochemistry·J W MuldersW M de Vos
Nov 1, 1969·Journal of Bacteriology·J Lopes, W E Inniss
Sep 1, 1993·FEMS Microbiology Reviews·T R Klaenhammer
Apr 1, 1996·The Journal of Applied Bacteriology·C E Wolf, W R Gibbons
Jan 22, 1998·Applied and Environmental Microbiology·A D Crandall, T J Montville
May 26, 1998·International Journal of Food Microbiology·P ZapicoM Nuñez
Oct 4, 2000·International Journal of Food Microbiology·I M Helander, T Mattila-Sandholm
Oct 12, 2000·International Journal of Food Microbiology·A NykänenA Lapveteläinen
Feb 20, 2002·Chemical Reviews·Mohamed A. MarahielHenning D. Mootz
Feb 8, 2006·Applied and Environmental Microbiology·Ruth E WirawanJohn R Tagg
Oct 31, 2006·Applied and Environmental Microbiology·Zengguo HeAhmed E Yousef
Jun 21, 2007·Journal of Applied Microbiology·K NaghmouchiI Fliss
Dec 18, 2007·International Journal of Antimicrobial Agents·Ahmad Z Sahalan, Ronald A Dixon

❮ Previous
Next ❯

Citations

Nov 1, 2011·Archives of Microbiology·Karim NaghmouchiDjamel Drider
Jan 25, 2012·Journal of Applied Microbiology·A D van StadenL M T Dicks
Jun 24, 2014·Annals of the New York Academy of Sciences·Heather K AllenThomas A Casey
Apr 22, 2015·International Journal of Food Microbiology·Lorena Rodríguez-RubioBeatriz Martínez
Jan 23, 2015·Archives of Microbiology·Fatoumata TambadouRomain Chevrot
Dec 19, 2015·Journal of Applied Microbiology·J M ShinY L Kapila
Aug 3, 2016·Probiotics and Antimicrobial Proteins·Djamel DriderMichael L Chikindas
Aug 26, 2016·Probiotics and Antimicrobial Proteins·Ahmed K Al AtyaDjamel Drider
Dec 21, 2014·Applied and Environmental Microbiology·Santosh Kumar TiwariMichael L Chikindas
May 1, 2018·NPJ Biofilms and Microbiomes·Harsh MathurR Paul Ross
Oct 30, 2020·International Journal of Environmental Research and Public Health·Hassan ZgheibYanath Belguesmia

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.