Nisin, a potent bacteriocin and anti-bacterial peptide, attenuates expression of metastatic genes in colorectal cancer cell lines

Microbial Pathogenesis
Zohreh NorouziHossein Fahimi

Abstract

Colorectal cancer is the third most common cause of cancer-related death in the world which genetic and environmental agents are responsible for cancer. When cells detach from the tumor and invade surrounding tissues, the tumor is malignant and may form secondary tumors at other locations in a process called metastasis. Probiotics are the largest group of inhabitation bacteria in the colon. Gut microbiota has a central role in prevented the risk colon cancer. Probiotics are beneficial microorganisms, like Lactic acid bacteria and Lactobacilli bacteria which are using in the dairy industry. Probiotics nisin are having the most important category of safe usage. In this study LS180, SW48, HT29 and Caco2 was cultured and treated with different dose of nisin. Cell proliferation was assayed with MTT. The expression of CEA, CEAM6 and MMP2F genes was analyzed with Real-time PCR. Protein expression of CEA was evacuated with ELISA. Our result was shown that the 40-50 IU/mL nisin could suppress proliferation of LS180. Cell proliferation of SW48, HT29, Caco2 cells was decreased in 250-350 IU/mL concentration of nisin. The gene expression of CEA, CEAM6, MMP2F was significantly down-regulated with nisin treatment (p < 0.001, p < 0.01). Also,...Continue Reading

Citations

Mar 5, 2020·International Journal of Colorectal Disease·Atieh YaghoubiSaman Soleimanpour
May 10, 2020·Cancers·Purushottam LamichhaneRahul R Deshmukh
Jun 12, 2020·Journal of Microbiology and Biotechnology·Chao Chen, Huajun Li
Dec 22, 2020·Biochimica Et Biophysica Acta. Reviews on Cancer·Aja BadgeleyAshakumary Lakshmikuttyamma
Mar 7, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Sonia ChauhanSimran Preet
May 4, 2021·Oxidative Medicine and Cellular Longevity·Fuqing HuangJin Zhong
Jun 3, 2021·Microorganisms·Edgar Torres-MaravillaLuis G Bermúdez-Humarán
Jul 21, 2021·BMC Microbiology·Sara EbrahimzadehShirin Eyvazi
Aug 10, 2020·Mini Reviews in Medicinal Chemistry·Zhi-Gang SunXin Ming
Oct 29, 2020·Current Molecular Medicine·Hadis FathizadehHossein Samadi Kafil

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