PMID: 8604128Mar 1, 1996Paper

Nitrate and nitrite-mediated transcription antitermination control of nasF (nitrate assimilation) operon expression in Klebsiella pheumoniae M5al

Journal of Molecular Biology
J T Lin, V Stewart

Abstract

Klebsiella pneumoniae can use nitrate and nitrite as sole nitrogen sources during aerobic growth. Nitrate is converted through nitrite to ammonium by assimilatory nitrate and nitrite reductase, respectively. Enzymes required for nitrate assimilation are encoded by the nasFEDCBA operon of K. pneumoniae; nasF operon expression is subject to both general nitrogen control and pathway-specific nitrate/nitrite induction, mediated by the NtrC and NasR proteins, respectively. Sequence inspection revealed a presumptive sigmaN (sigma54)-dependent promoter as well as two presumptive upstream NtrC protein binding sites. Site-specific mutational and primer extension analyses confirmed the identity of the sigmaN-dependent promoter. Deletions removing the apparent NtrC protein binding sites greatly reduced NtrC-dependent regulation, indicating that these sites are involved in general nitrogen control. However, deletions removing most of the sequence upstream of the promoter had little effect on nitrate/nitrite regulation, suggesting that the nasF leader region is involved in nitrate/nitrite regulation. The 119 nucleotide long transcribed leader region contains an apparent factor-independent transcription terminator. Promoter replacement exper...Continue Reading

Citations

Nov 21, 2007·Biochemistry. Biokhimii︠a︡·E V Morozkina, R A Zvyagilskaya
Jun 12, 2012·PLoS Genetics·Valley Stewart, Herman van Tilbeurgh
Sep 6, 2013·The Journal of Biological Chemistry·Victor M Luque-AlmagroAndrew J Gates
Jan 19, 2006·Biochemical Society Transactions·C PinoC Moreno-Vivián
Oct 27, 2001·Applied and Environmental Microbiology·A E AllenS Zani
Nov 26, 2008·Water Environment Research : a Research Publication of the Water Environment Federation·William C Hiatt, C P Leslie Grady
Jul 22, 2018·Microbiology Spectrum·Daniela Prasse, Ruth A Schmitz

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