Nitrate assimilation contributes to Ralstonia solanacearum root attachment, stem colonization, and virulence

Journal of Bacteriology
Beth L Dalsing, C Allen

Abstract

Ralstonia solanacearum, an economically important plant pathogen, must attach, grow, and produce virulence factors to colonize plant xylem vessels and cause disease. Little is known about the bacterial metabolism that drives these processes. Nitrate is present in both tomato xylem fluid and agricultural soils, and the bacterium's gene expression profile suggests that it assimilates nitrate during pathogenesis. A nasA mutant, which lacks the gene encoding the catalytic subunit of R. solanacearum's sole assimilatory nitrate reductase, did not grow on nitrate as a sole nitrogen source. This nasA mutant exhibited reduced virulence and delayed stem colonization after soil soak inoculation of tomato plants. The nasA virulence defect was more severe following a period of soil survival between hosts. Unexpectedly, once bacteria reached xylem tissue, nitrate assimilation was dispensable for growth, virulence, and competitive fitness. However, nasA-dependent nitrate assimilation was required for normal production of extracellular polysaccharide (EPS), a major virulence factor. Quantitative analyses revealed that EPS production was significantly influenced by nitrate assimilation when nitrate was not required for growth. The plant coloniz...Continue Reading

References

Sep 1, 1990·Molecular Plant-microbe Interactions : MPMI·D L Coplin, D Cook
Dec 1, 1990·Journal of Bacteriology·R A Bender, B Friedrich
Jun 2, 1994·Carbohydrate Research·N A KocharovaN V Moskalenko
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·J Graf, E G Ruby
Apr 6, 2001·Cellular and Molecular Life Sciences : CMLS·J W Moir, N J Wood
May 23, 2002·Applied Biochemistry and Biotechnology·Alexandre S SantosDenise M G Freire
May 24, 2005·Infection and Immunity·M J FiliatraultL Passador
Jul 1, 1984·Applied and Environmental Microbiology·C A Hendrick, L Sequeira
Jul 1, 1992·Applied and Environmental Microbiology·M A Jackson, D A Schisler
Jan 13, 2006·Molecular Plant-microbe Interactions : MPMI·Dean W GabrielNatalia Mikhailova
Feb 27, 2008·Journal of Applied Microbiology·H T WattanaphonA S Vangnai
Jan 15, 2010·Cellular Microbiology·Allan Jefferson GuimarãesJoshua D Nosanchuk
Oct 25, 2011·Cell Host & Microbe·Michael A Fischbach, Justin L Sonnenburg
Nov 23, 2011·Biochemical Society Transactions·Víctor M Luque-AlmagroM Dolores Roldán
May 9, 2012·Annual Review of Phytopathology·Stéphane Genin, Timothy P Denny
Nov 20, 2012·Applied and Environmental Microbiology·Audrey Parangan-Smith, Steven Lindow

❮ Previous
Next ❯

Citations

Nov 24, 2016·Environmental Microbiology·Simon LeonardSylvie Reverchon
Nov 21, 2017·Molecular Plant-microbe Interactions : MPMI·Elizabeth FrenchAnjali S Iyer-Pascuzzi
Mar 14, 2019·Scientific Reports·Manish KumarIndu Shekhar Thakur
Apr 5, 2017·Frontiers in Plant Science·Marina PuigvertMarc Valls
Nov 26, 2019·Molecular Plant-microbe Interactions : MPMI·April M MacIntyreCaitilyn Allen
Sep 20, 2018·Bio-protocol·Devanshi KhokhaniCaitilyn Allen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.