PMID: 8990301Jan 1, 1997Paper

Nitrate assimilation gene cluster from the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120

Journal of Bacteriology
J E FríasA Herrero

Abstract

A region of the genome of the filamentous, nitrogen-fixing, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120 that contains a cluster of genes involved in nitrate assimilation has been identified. The genes nir, encoding nitrite reductase, and nrtABC, encoding elements of a nitrate permease, have been cloned. Insertion of a gene cassette into the nir-nrtA region impaired expression of narB, the nitrate reductase structural gene which together with nrtD is found downstream from nrtC in the gene cluster. This indicates that the nir-nrtABCD-narB genes are cotranscribed, thus constituting an operon. Expression of the nir operon in strain PCC 7120 is subjected to ammonium-promoted repression and takes place from an NtcA-activated promoter located 460 bp upstream from the start of the nir gene. In the absence of ammonium, cellular levels of the products of the nir operon are higher in the presence of nitrate than in the absence of combined nitrogen.

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Citations

Jan 26, 2005·Biochemical Society Transactions·E Flores, A Herrero
Jan 19, 2006·Biochemical Society Transactions·C PinoC Moreno-Vivián
Dec 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jan MitschkeAlicia M Muro-Pastor
Aug 22, 2014·Environmental Microbiology·Paulo OliveiraPaula Tamagnini
Dec 4, 2003·Molecular Genetics and Genomics : MGG·G MazónJ Barbé
Jun 26, 2009·BMC Bioinformatics·Ziomara P GerdtzenBarbara A Andrews
May 24, 2011·Journal of Bacteriology·Elena Puerta-Fernández, Agustín Vioque
Apr 19, 2012·PLoS Computational Biology·João F Matias RodriguesHomayoun C Bagheri
Nov 21, 2007·Biochemistry. Biokhimii︠a︡·E V Morozkina, R A Zvyagilskaya
Jun 22, 2016·Plant Molecular Biology·Mareike RudolfEnrico Schleiff
Aug 2, 2018·Environmental Microbiology·Antonia Herrero, Enrique Flores
Sep 7, 2005·Photosynthesis Research·Enrique FloresAntonia Herrero
Oct 7, 2008·Journal of Bacteriology·Douglas D Risser, Sean M Callahan
Feb 15, 2000·Journal of Bacteriology·E Hoiczyk, A Hansel
Jan 3, 2001·Journal of Bacteriology·A HerreroE Flores
Feb 5, 2013·Biochimica Et Biophysica Acta·Stéphane GrimaldiAxel Magalon

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