PMID: 11926996Apr 3, 2002Paper

Nitrate reductase-formate dehydrogenase couple involved in the fungal denitrification by Fusarium oxysporum

Journal of Biochemistry
Hiromasa UchimuraHirofumi Shoun

Abstract

Dissimilatory nitrate reductase (Nar) was solubilized and partially purified from the large particle (mitochondrial) fraction of the denitrifying fungus Fusarium oxysporum and characterized. Many lines of evidence showed that the membrane-bound Nar is distinct from the soluble, assimilatory nitrate reductase. Further, the spectral and other properties of the fungal Nar were similar to those of dissimilatory Nars of Escherichia coli and denitrifying bacteria, which are comprised of a molybdoprotein, a cytochrome b, and an iron-sulfur protein. Formate-nitrate oxidoreductase activity was also detected in the mitochondrial fraction, which was shown to arise from the coupling of formate dehydrogenase (Fdh), Nar, and a ubiquinone/ubiquinol pool. This is the first report of the occurrence in a eukaryote of Fdh that is associated with the respiratory chain. The coupling with Fdh showed that the fungal Nar system is more similar to that involved in the nitrate respiration by Escherichia coli than that in the bacterial denitrifying system. Analyses of the mutant species of F. oxysporum that were defective in Nar and/or assimilatory nitrate reductase conclusively showed that Nar is essential for the fungal denitrification.

Citations

Mar 28, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Hirofumi ShounTakayoshi Wakagi
Sep 16, 2008·Applied and Environmental Microbiology·W K MaS D Siciliano
Mar 10, 2009·Applied and Environmental Microbiology·Sang-Wan KimHirofumi Shoun
Jun 13, 2012·Microbiology and Molecular Biology Reviews : MMBR·Miklós MüllerWilliam F Martin
Nov 21, 2007·Biochemistry. Biokhimii︠a︡·E V Morozkina, R A Zvyagilskaya
Oct 20, 2005·Journal of Bioscience and Bioengineering·Naoki Takaya
Jul 2, 2003·Bioscience, Biotechnology, and Biochemistry·Tomo-o WatsujiHirofumi Shoun
Jul 2, 2003·Bioscience, Biotechnology, and Biochemistry·Tomo-o WatsujiHirofumi Shoun
Jun 6, 2003·Bioscience, Biotechnology, and Biochemistry·Seigo KuwazakiHirofumi Shoun
Oct 12, 2007·Bioscience, Biotechnology, and Biochemistry·Tsuyoshi AbeNaoki Takaya
Feb 8, 2008·Bioscience, Biotechnology, and Biochemistry·Tatsuya Fujii, Naoki Takaya
Jan 9, 2009·Bioscience, Biotechnology, and Biochemistry·Naoki Takaya
May 13, 2010·Bioscience, Biotechnology, and Biochemistry·Yoshito NakanishiHirofumi Shoun
Mar 31, 2012·Annual Review of Marine Science·Thomas A RichardsDavid Bass
Nov 28, 2007·Prikladnaia biokhimiia i mikrobiologiia·E V Morozkina, A V Kurakov
Dec 3, 2015·Antonie van Leeuwenhoek·Sean C ThomasVasu D Appanna
Mar 15, 2016·Frontiers in Plant Science·Magdalena Arasimowicz-Jelonek, Jolanta Floryszak-Wieczorek
May 14, 2014·Molecular Plant Pathology·Magdalena Arasimowicz-Jelonek, Jolanta Floryszak-Wieczorek
Jan 25, 2017·Journal of Chemical Information and Modeling·Masaki MatsuokaKam Y J Zhang
Jun 18, 2019·Journal of the Science of Food and Agriculture·Monalisa RayShikha Singh

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