Nitration of the pollen allergen bet v 1.0101 enhances the presentation of bet v 1-derived peptides by HLA-DR on human dendritic cells.

PloS One
Anette C KarleAlbert Duschl

Abstract

Nitration of pollen derived allergens can occur by NO(2) and ozone in polluted air and it has already been shown that nitrated major birch (Betula verrucosa) pollen allergen Bet v 1.0101 (Bet v 1) exhibits an increased potency to trigger an immune response. However, the mechanisms by which nitration might contribute to the induction of allergy are still unknown. In this study, we assessed the effect of chemically induced nitration of Bet v 1 on the generation of HLA-DR associated peptides. Human dendritic cells were loaded with unmodified Bet v 1 or nitrated Bet v 1, and the naturally processed HLA-DR associated peptides were subsequently identified by liquid chromatography-mass spectrometry. Nitration of Bet v 1 resulted in enhanced presentation of allergen-derived HLA-DR-associated peptides. Both the copy number of Bet v 1 derived peptides as well as the number of nested clusters was increased. Our study shows that nitration of Bet v 1 alters antigen processing and presentation via HLA-DR, by enhancing both the quality and the quantity of the Bet v 1-specific peptide repertoire. These findings indicate that air pollution can contribute to allergic diseases and might also shed light on the analogous events concerning the nitra...Continue Reading

References

Feb 1, 1994·Biological Chemistry Hoppe-Seyler·J S BeckmannC R White
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S FatenejadJ Craft
Jul 1, 1997·Journal of Neuroimmunology·R C van der VeenF M Hofman
May 26, 1998·Journal of Neuropathology and Experimental Neurology·P F GoodC W Olanow
Aug 4, 1998·Journal of Neuroimmunology·A H CrossT P Misko
Jul 31, 1999·The Journal of Biological Chemistry·M J MamulaP R Blier
Nov 5, 1999·Archives of Biochemistry and Biophysics·J M SouzaH Ischiropoulos
Aug 10, 2000·Archives of Biochemistry and Biophysics·J M SouzaH Ischiropoulos
Jul 28, 2001·Trends in Immunology·H A Doyle, M J Mamula
Jan 5, 2002·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·J RingH Behrendt
Jul 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·H Chaim BirnboimJohn R Webb
Jul 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jonathan A HillEwa Cairns
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Mar 8, 2005·The Journal of Biological Chemistry·Catherine X MossColin Watts
Apr 12, 2005·Environmental Science & Technology·Thomas FranzeUlrich Pöschl
May 17, 2005·Autoimmunity Reviews·Hitoshi Ohmori, Naoki Kanayama
Oct 14, 2005·Epidemiology·W James GaudermanRob McConnell
Dec 14, 2005·Current Opinion in Immunology·Victor H EngelhardAngela L Zarling
Jan 31, 2006·Free Radical Biology & Medicine·Helen R GriffithsHilary J Powers
May 6, 2006·Environmental Health Perspectives·Rob McConnellJohn Peters
May 30, 2006·The International Journal of Biochemistry & Cell Biology·Bence GyörgyEdit I Buzás
Aug 26, 2006·International Archives of Allergy and Immunology·Y K GruijthuijsenA Duschl
Nov 30, 2006·Current Opinion in Pulmonary Medicine·Jeremy A Sarnat, Fernando Holguin
Nov 6, 2007·Immunological Reviews·Shin-Ichiro FujiiRalph M Steinman
Jul 22, 2008·Nature Reviews. Immunology·Jatin M VyasHidde L Ploegh
Feb 6, 2010·The Journal of Allergy and Clinical Immunology·Sonja MutschlechnerFatima Ferreira
Jul 20, 2010·The Journal of Allergy and Clinical Immunology·Beatrice Jahn-SchmidBarbara Bohle
Jan 25, 2011·Molecular BioSystems·Zexian LiuYu Xue

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Citations

Feb 13, 2016·Frontiers in Plant Science·Ulrike Frank, Dieter Ernst
May 29, 2015·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Hugo GonzálezRodrigo Pacheco
Aug 20, 2015·Environmental Science & Technology·Christopher J KampfUlrich Pöschl
May 3, 2014·Journal of Toxicology and Environmental Health. Part a·Renate HochscheidBernd Müller
Jan 3, 2014·International Journal of Environmental Health Research·Helena RibeiroIlda Abreu
Jun 1, 2017·Immunity, Inflammation and Disease·Laetitia SordéAnette Karle
Dec 25, 2016·Journal of Neuroimmunology·Andreas WarneckeRobert A Harris
Dec 19, 2015·The European Respiratory Journal·Antoine FroidureCharles Pilette
Sep 17, 2013·Analytical and Bioanalytical Chemistry·Kathrin SelzleUlrich Pöschl
Jul 15, 2016·Analytical and Bioanalytical Chemistry·Fobang LiuChristopher J Kampf
May 13, 2019·Environmental Monitoring and Assessment·Fateme MousaviFarrokh Ghahremaninejad
Jun 8, 2017·International Journal of Molecular Sciences·Barbara HöllbacherPeter Lackner
May 7, 2020·Frontiers in Immunology·Anette C Karle
Nov 8, 2017·Environmental Science & Technology·Manabu ShiraiwaKei Sato

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Methods Mentioned

BETA
affinity purification
X-ray
size exclusion chromatography
Protein assay
density gradient centrifugation

Software Mentioned

SPSS
GPS
MudPIT
- Chimera
Chimera
UCSF
DelPhi

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