Nitrergic innervation and relaxant response of rectal circular smooth muscle

Diseases of the Colon and Rectum
J F StebbingN J Mortensen

Abstract

This study was designed to investigate whether nitric oxide mediates inhibitory innervation in human rectal circular smooth muscle. Tissue was obtained from the midrectum of patients undergoing anterior resection for carcinoma. Adjacent strips of circular muscle were dissected and mounted in superfusion organ baths for isometric tension recording and initially loaded with 1 g of weight. Strips were continuously bathed with standard Krebs solution (37 degrees C, bubbled with 97 percent O2/3 percent CO2) containing 3 X X 10(-6) M guanethidine and 3 X 10(-6) M atropine sulfate to block adrenergic and muscarinic cholinergic neurotransmission. After equilibration, strips had no intrinsic tone, and reproducible and stable tension was, therefore, induced by the addition of 3 X 10 M histamine for five-minute "test" periods, during which electrical field stimulation (EFS) and additional drugs were applied. EFS elicited frequency-dependent, neurogenic (tetrodotoxin-sensitive) relaxations of precontracted strips. Addition of N-w-nitro-L-arginine, a powerful competitive inhibitor of nitric oxide synthase, reduced the relaxant response to EFS in a dose-dependent fashion, and effect reversed by addition of s X 10(-4) M L-arginine but not by ...Continue Reading

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Citations

Jul 1, 1997·Diseases of the Colon and Rectum·H BacherW Schweiger
Jun 23, 1999·Diseases of the Colon and Rectum·T A CookN J Mortensen
Jan 9, 2009·Amino Acids·J Marc Rhoads, Guoyao Wu
Mar 5, 2005·Diseases of the Colon and Rectum·M P GosselinkW R Schouten
Jun 3, 2008·Diseases of the Colon and Rectum·Flemming V MøllerSøren Laurberg
Aug 3, 2002·The British Journal of Surgery·G MariaG Brisinda
Jun 11, 1999·The American Journal of Gastroenterology·L SiproudhisM Gosselin
Jun 1, 1997·Diseases of the Colon and Rectum·J F StebbingN J Mortensen

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