PMID: 2497225May 1, 1989Paper

Nitric oxide. A macrophage product responsible for cytostasis and respiratory inhibition in tumor target cells

The Journal of Experimental Medicine
D J Stuehr, C F Nathan

Abstract

A metabolic pathway of activated macrophages (M phi) involving oxidation of the guanido nitrogens of L-arginine is required for inhibition of growth and respiration of some target cells. The goal of this study was to identify the M phi metabolite(s) that induce these injuries. The stable products of the L-arginine pathway, NO2- and NO3-, were incapable of causing cytostasis under coculture conditions. However, NO2- became cytostatic upon mild acidification, which favors its transformation into nitrogen oxides of greater reactivity. This suggested that NO. (and/or NO2), recently identified as an M phi metabolite of L-arginine, could be a mediator. Authentic NO. caused cytostasis and respiratory inhibition in L1210 cells in a dose-dependent manner. The mitochondrial lesions caused by NO. were confined to complex 1 and 2, a pattern of injury identical to that seen after coculture with activated M phi. Inclusion of NO. scavenger systems prevented cytostasis from developing in M phi-L1210 cocultures. Thus, M phi-generated NO. can account for L-arginine-dependent cytostasis and respiratory inhibition.

References

Nov 23, 1979·Biochimica Et Biophysica Acta·H KosakaI Tyuma
Aug 1, 1976·Cellular Immunology·J L KrahenbuhlJ S Remington
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R IyengarM A Marletta
Apr 1, 1989·The Journal of Experimental Medicine·T R BilliarR L Simmons
Jul 1, 1985·The Journal of Clinical Investigation·J O'Donnell-TormeyC F Nathan
Nov 30, 1988·Biochemical and Biophysical Research Communications·J B HibbsE M Rachlin
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J L UrbanH Schreiber
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D J Stuehr, M A Marletta
Jun 5, 1970·Biochemical and Biophysical Research Communications·C D Lytle, W Ginoza
Jan 1, 1984·Annual Review of Immunology·D O Adams, T A Hamilton
Nov 1, 1982·The Journal of Cell Biology·D L Granger, A L Lehninger
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L C GreenS R Tannenbaum
Feb 1, 1980·The Journal of Clinical Investigation·D L GrangerJ B Hibbs

❮ Previous
Next ❯

Citations

Feb 7, 1997·International Journal of Cancer. Journal International Du Cancer·E FontanR M Fauve
Jan 14, 1998·International Journal of Cancer. Journal International Du Cancer·T YamamotoH Kosaka
Apr 1, 1996·Journal of Cellular Physiology·R D Hurst, I B Fritz
Jun 1, 1997·Journal of Cellular Physiology·K J GoochJ A Frangos
Oct 25, 2000·The Journal of Gene Medicine·M N SolerS Lausson
Sep 1, 1992·Annals of Neurology·T M DawsonS H Snyder
Jul 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J C Drapier, C Bouton
Jul 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S H RalstonN Benjamin
May 1, 1994·Journal of Cellular Physiology·M Stefanovic-RacicC H Evans
Jan 1, 1994·Medicinal Research Reviews·J F Kerwin, M Heller
Apr 25, 2000·Biochemical and Biophysical Research Communications·S M HwangJ C Chen
Dec 9, 2000·Biochemical and Biophysical Research Communications·J H YinC Y Hsu
Oct 12, 2001·Biochemical and Biophysical Research Communications·C Y ChoiH G Jeong
Jun 1, 1996·Cancer Metastasis Reviews·V UmanskyV Schirrmacher
Jan 1, 1992·Cancer Immunology, Immunotherapy : CII·K Ryoyama
Jan 1, 1991·Cancer Immunology, Immunotherapy : CII·M TakemaS Muramatsu
Jan 1, 1993·Agents and Actions·A R BaydounG E Mann
Jan 1, 2002·Environmental Health and Preventive Medicine·Takako KizakiHideki Ohno
Jan 1, 1996·Immunologic Research·W Krenger, J L Ferrara
May 10, 2001·International Journal of Hematology·S SuzuK Motoyoshi
Feb 8, 2005·Cancer Immunology, Immunotherapy : CII·Katrin U LundinBjarne Bogen
Jun 29, 2004·Cancer Immunology, Immunotherapy : CII·Marina BacacSabrina Pacor

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.