Nitric oxide and excitatory postsynaptic currents in immature rat sympathetic preganglionic neurons in vitro

Neuroscience
S Y WuN J Dun

Abstract

Neuronal nitric oxide synthase immunoreactivity was localized to sympathetic preganglionic neurons of the intermediolateral cell column and cyclic GMP immunoreactivity to nerve fibers projecting into the intermediolateral cell column of 20-25-day-old rats. Whole-cell patch-clamp recordings were made from sympathetic preganglionic neurons in spinal cord slices of immature rats and the role of nitric oxide and cyclic GMP on excitatory postsynaptic currents was studied. Superfusing the slices with the nitric oxide precursor L-arginine (300 microM) increased the amplitude of evoked excitatory postsynaptic currents as well as the frequency of spontaneous miniature excitatory postsynaptic currents in some neurons from minutes to over 1 h. The nitric oxide synthase inhibitor N(W)-nitro-L-arginine (100 microM) and the nitric oxide scavenger hemoglobin (100 microM) antagonized the potentiating effect of L-arginine. The nitric oxide donor sodium nitroprusside (100 microM) potentiated the synaptic currents in a manner similar to that of L-arginine and this effect was blocked by hemoglobin. The membrane-permeable cyclic GMP analogue dibutyryl guanosine 3',5'-cyclic monophosphate (350 microM), in the presence of the phosphodiesterase inhibi...Continue Reading

References

Apr 25, 1977·Biochemical and Biophysical Research Communications·N MikiK Kuriyama
Dec 7, 1992·Neuroscience Letters·N J DunL F Tseng
Jul 8, 1992·The Journal of Comparative Neurology·J G ValtschanoffA Rustioni
Sep 22, 1992·European Journal of Pharmacology·G LonartK M Johnson
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T J O'DellO Arancio
May 15, 1991·Biochemical and Biophysical Research Communications·M A DwyerS H Snyder
Jul 9, 1991·European Journal of Pharmacology·G A BöhmeJ C Blanchard
Nov 1, 1993·Neuropharmacology·E Southam, J Garthwaite
Mar 1, 1994·Neuroscience·N J DunU Förstermann
Mar 1, 1994·Neuroscience·S SaitoD F Hanley
Sep 1, 1993·Clinical and Experimental Hypertension : CHE·A O el KaribR L Malvin
Mar 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·R WettsJ E Vaughn

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Citations

Nov 10, 2001·Brain Research·M L WeissJ Huang
Jul 17, 1999·Brain Research. Brain Research Reviews·T L Krukoff
Nov 26, 2010·Pflügers Archiv : European journal of physiology·João Paulo J SabinoHelio C Salgado
Sep 3, 2009·The Journal of Physiology·Colin N YoungPaul J Fadel
Jul 1, 2008·The European Journal of Neuroscience·John Garthwaite
Aug 21, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Cristiano BombardiBruno Cozzi
Nov 24, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·W A SaadS Simões
Aug 14, 2020·Journal of Anatomy·Ieva NavickaiteDainius H Pauza
Feb 13, 2001·American Journal of Physiology. Heart and Circulatory Physiology·H C SouzaH C Salgado
Nov 16, 2002·Journal of Applied Physiology·Emmanuel M MbakuJohn Buchholz
Feb 4, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yoshitaka HirookaKenji Sunagawa

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