PMID: 9441905Aug 1, 1997Paper

Nitric oxide and peroxynitrite affect differently acetylcholine release, choline acetyltransferase activity, synthesis, and compartmentation of newly formed acetylcholine in Torpedo marmorata synaptosomes

Nitric Oxide : Biology and Chemistry
Y Morot Gaudry-TalarmainC Ducrocq

Abstract

Recent reports proposed that nitric oxide was a modulator of cholinergic transmission. Here, we examined the role of NO on cholinergic metabolism in a model of the peripheral cholinergic nervous synapse: synaptosomes from Torpedo electric organ. The presence of NO synthase was immunodetected in the cell bodies, in the nerve ending area of nerve-electroplate tissue and in the electroplates. Exogenous source of NO was provided from SIN1, a donor of NO and O2-., and an end-derivative peroxynitrite (ONOO-). SIN1 increased calcium-dependent acetylcholine (ACh) release induced by KCl depolarization or a calcium ionophore A23187. The formation of ONOO- was continuously followed by a new chemiluminescent assay. The addition of superoxide dismutase, that decreases the formation of ONOO-, did not impair the stimulation of ACh release, suggesting that NO itself was the main stimulating agent. When the endogenous source of NO was blocked by proadifen, an inhibitor of cytochrome P450 activity of NO synthase, both KCl- and A23187-induced ACh release were abolished; nevertheless, the inhibitor Ng-monomethyl-L-arginine did not modify ACh release when applied in a short time duration of action. Both NO synthase inhibitors reduced the synthesis ...Continue Reading

References

Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·V M VergeT Hökfelt
Jun 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C VillalobosJ García-Sancho
Jul 8, 1992·The Journal of Comparative Neurology·J G ValtschanoffA Rustioni
May 27, 1992·European Journal of Pharmacology·H Prast, A Philippu
Nov 1, 1992·Archives of Biochemistry and Biophysics·H IschiropoulosJ S Beckman
Sep 22, 1992·European Journal of Pharmacology·G LonartK M Johnson
May 1, 1992·Chemical Research in Toxicology·J J Moreno, W A Pryor
Jun 6, 1991·European Journal of Pharmacology·M G BelvisiP J Barnes
Mar 1, 1991·Archives of Biochemistry and Biophysics·R TakahashiK Utsumi
Aug 1, 1991·Archives of Biochemistry and Biophysics·R RadiB A Freeman
Nov 1, 1990·Neurology·H J KaminskiR L Ruff
Feb 1, 1991·Trends in Neurosciences·J Garthwaite
Sep 1, 1990·Trends in Pharmacological Sciences·D Piomelli, P Greengard
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J S BeckmanB A Freeman
Sep 1, 1990·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·H OhshimaH Bartsch
Apr 1, 1994·Journal of Neurochemistry·M HeckerR Busse
May 1, 1994·Naunyn-Schmiedeberg's Archives of Pharmacology·H Kilbinger, D Wolf
Dec 8, 1994·Nature·L KobzikJ S Stamler
Nov 1, 1995·Journal of Neurochemistry·H IschiropoulosJ Horwitz
Oct 1, 1993·British Journal of Pharmacology·C U WiklundL E Gustafsson
May 15, 1995·FEBS Letters·H Ischiropoulos, A B al-Mehdi
Feb 1, 1994·Journal of Neurochemistry·R Guevara-GuzmanK M Kendrick
Dec 1, 1994·The Journal of Clinical Investigation·I Y HaddadS Matalon
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·E M SchumanD V Madison
May 1, 1994·Archives of Biochemistry and Biophysics·N W Kooy, J A Royall
Oct 1, 1993·British Journal of Pharmacology·K SekizawaH Sasaki

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Citations

Oct 31, 2006·European Journal of Protistology·Andrea AmaroliMaria Umberta Delmonte Corrado
Feb 27, 2016·The Journal of Physiology·Dan Li, David J Paterson
Jul 16, 2008·Chemical Reviews·Christian AmatoreFrédéric Lemaître

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