Nitric oxide and peroxynitrite induce cellular death in bovine chromaffin cells: evidence for a mixed necrotic and apoptotic mechanism with caspases activation

Journal of Neuroscience Research
S VicenteM J Oset-Gasque

Abstract

Treatment of chromaffin cells with nitric oxide (NO) donors (SNP and SNAP) and peroxynitrite produces a time- and dose-dependent necrotic and apoptotic cell death. Necrotic cell death was characterized by both an increase in lactate dehydrogenase and ATP release and changes in nuclei and cell morphology (as seen with fluorescence microscopy analysis with propidium iodide and Hoechst 33342). Apoptotic cell death was characterized by nuclear fragmentation and presence of apoptotic cell bodies, by a decrease in DNA content, and by an increase in DNA fragmentation. Treatment of chromaffin cells with lipopolysaccharide (LPS) or cytokines (interferon-gamma, tumor necrosis factor-alpha) resulted only in apoptotic cell death. Apoptotic effects of NO-inducing compounds were specifically reversed, depending on the stimuli, by the NO scavenger carboxy-PTIO (CPTio) or by the NOS inhibitors L-NMA and thiocitrulline. NO-induced apoptotic death in chromaffin cells was concomitant to a cell cycle arrest in G0G1 phase and a decrease in the number of chromaffin cells in the G2M and S phases of cell cycle. All NO-producing compounds were able to induce activation of caspase 3 and cytochrome c release, and specific inhibitors of caspase 3 and 9, s...Continue Reading

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Citations

Jun 27, 2007·Neurochemical Research·Christopher Brynczka, Bruce Alex Merrick
Jun 19, 2007·Antioxidants & Redox Signaling·Masahiro OkouchiTak Yee Aw
Oct 31, 2012·International Journal of Molecular Sciences·Mustafa KassimKamaruddin Mohd Yusoff
Mar 27, 2007·Free Radical Biology & Medicine·Kota V RamanaSatish K Srivastava
Jan 15, 2009·Journal of Neurochemistry·Rocío Pérez-RodríguezMaría Jesús Oset-Gasque
Nov 4, 2010·Journal of Neuroscience Research·A M OlivánM J Oset-Gasque
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May 25, 2007·Journal of Neuroscience Research·Rocío Pérez-RodríguezMaría J Oset-Gasque
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Oct 30, 2020·International Journal of Molecular Sciences·Daniel Diez-IriepaMaría Jesús Oset-Gasque
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Beatriz ChamorroMaría Jesús Oset-Gasque
Aug 29, 2019·ACS Omega·Dorota G PiotrowskaMaría Jesús Oset-Gasque

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