PMID: 8960707Nov 1, 1996Paper

Nitric oxide and peroxynitrite released by ultraviolet B-irradiated human endothelial cells are possibly involved in skin erythema and inflammation

Experimental Physiology
G DeliconstantinosJ C Stavrides

Abstract

In this study we attempted to demonstrate whether endothelial cell nitric oxide synthase (eNOS) and xanthine oxidase (XO) could be activated to release nitric oxide (NO) and peroxynitrite (ONOO-) following exposure to ultraviolet B (UVB) radiation and to define whether this light-induced response could be involved in the pathogenesis of sunburn erythema and inflammation. Treatment of human endothelial cells with UVB (290-320 nm) radiation (up to 100 mJ/cm2) resulted in an increase of both NO and ONOO- release that was inhibited by NG-monomethyl-L-arginine (L-NMMA). Treatment of cell cytosol with various doses of UVB radiation (up to 20 mJ/cm2) resulted in a threefold increase of XO activity that was inhibited (approximately 90% by oxypurinol. In reconstitution experiments, when purified eNOS was added to purified XO, an almost fourfold increase in ONOO- production at 20 mj/cm2 UVB radiation was observed. UVB radiation (100 mg/cm2) decreased cell membrane fluidity, indicating changes in the physicochemical characteristics of the membranes. In in vivo experiments, when human volunteers were subjected to UVB light, a protection factor (PF) of 3.90 +/- 0.85 was calculated when an emulsified cream formulation containing nitro-L-argi...Continue Reading

Citations

Mar 31, 2000·Burns : Journal of the International Society for Burn Injuries·A RawlingsonS D Brain
Mar 7, 2002·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Antonella SaijaFrancesco Castelli
May 16, 2000·The British Journal of Dermatology·A D OrmerodS A Shah
Apr 11, 2001·Mutation Research·K J Claycombe, S N Meydani
Jun 16, 2010·Nitric Oxide : Biology and Chemistry·Wei Liu, Shiyong Wu
Nov 3, 2009·Nitric Oxide : Biology and Chemistry·Christoph V SuschekErnst E van Faassen
Mar 21, 2012·Molecular Carcinogenesis·Wei Lu, Shiyong Wu
Jul 28, 2004·Nitric Oxide : Biology and Chemistry·M-M Cals-Grierson, A D Ormerod
Jul 10, 2002·Experimental Dermatology·László VirágCsaba Szabó
Mar 22, 2000·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·T W Stief, J Fareed
Apr 26, 2005·International Journal of Pharmaceutics·C RossiA Rossi
Dec 1, 2004·Medicine and Science in Sports and Exercise·Ioannis G FatourosGeorge Deliconstantinos
Jan 1, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Dionisios MitropoulosVassilis G Gorgoulis

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