PMID: 8944635Nov 1, 1996Paper

Nitric oxide and superoxide anion production during endothelial cell proliferation

The American Journal of Physiology
J F ArnalA Nègre-Salvayre

Abstract

We have previously reported that nitric oxide (NO) synthase activity, protein, and mRNA are increased in proliferating compared with postconfluent bovine aortic endothelial cells (BAEC). Because superoxide anion inactivates NO, in the present study, we have assessed the effect of proliferation on superoxide anion production by use of cytochrome c reduction. The superoxide anion production in proliferating cells was increased about threefold compared with postconfluent cells in both basal and calcium ionophore-stimulated conditions and exceeded the amount of released nitrite and nitrate (NOx) in all cases. A-23187 (1 microM) stimulated the superoxide anion production about twofold at all stages of confluence. Because superoxide anion can inactivate NO, we then assessed the effect of proliferation on NO bioactivity released in the conditioned medium, by use of RFL-6 cells (reporter cells very rich in guanylate cyclase, which on activation by NO generates guanosine 3',5'-cyclic monophosphate, second messenger of NO). In the absence of added superoxide dismutase (SOD) in the conditioned medium, the guanylate cyclase-stimulating activities evoked by A-23187 from proliferating and growth-arrested cells were similar, despite a greater...Continue Reading

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Citations

Feb 7, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·M ZanettiT O'Brien
Aug 13, 2004·Vascular and Endovascular Surgery·Joseph L UnthankMichael C Dalsing
May 18, 2006·The FEBS Journal·Antonio Villalobo
Jun 10, 2011·European Journal of Applied Physiology·Kyriaki VenetsanouGeorge Baltopoulos
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Jul 27, 2000·Endothelium : Journal of Endothelial Cell Research·S ZöllnerG M Rubanyi
May 12, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ru-Jeng TengGirija G Konduri
Jun 17, 2005·American Journal of Physiology. Cell Physiology·Matias S Attene-RamosH Rex Gaskins
Dec 19, 2001·Microscopy Research and Technique·C L Murrant, M B Reid
Sep 18, 1999·Free Radical Biology & Medicine·Y TampoM Yonaha
Jan 23, 1998·Free Radical Biology & Medicine·C P TurnerO T Jones

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