Abstract
Lipopolysaccharide (LPS) is known to generate nitric oxide (NO) in the airway through the activation of nitric-oxide synthase (NOS). The functional consequences of this on the inflammatory response are not clear, with conflicting data published. In the clinic, exhaled NO (ex-NO) is used as a noninvasive biomarker to assess the extent of airway inflammation. It is proposed that monitoring levels of ex-NO could be a useful guide to determining the effectiveness of disease modifying therapies. The aim was, using pharmacological tools, to determine the role of NO in an aerosolized LPS-driven animal model of airway inflammation by assessment of ex-NO, neutrophilia, and inflammatory biomarkers, using a nonselective NOS inhibitor, N(G)-nitro-l-arginine methyl ester (l-NAME), and a selective inducible NOS (iNOS) inhibitor, N-3 (aminomethyl)benzyl)acetamidine (1400W). Real-time mRNA analysis of the lung tissue indicated an increased gene expression of iNOS following LPS challenge with minimal impact on constitutive NOS isoforms. LPS induced an increase in ex-NO, which appeared to correlate with the increase in iNOS gene expression and airway neutrophilia. Treatment with l-NAME and 1400W resulted in comparable reductions in ex-NO, a redu...Continue Reading
References
Oct 20, 1992·European Journal of Pharmacology·H P KuoP J Barnes
Nov 1, 1990·British Journal of Pharmacology·D D ReesS Moncada
Jul 1, 1994·The Journal of Investigative Dermatology·E DippelR Paus
Aug 1, 1995·Nature Medicine·J L ZweierP Kuppusamy
Oct 1, 1993·American Journal of Respiratory Cell and Molecular Biology·L KobzikJ S Stamler
Apr 1, 1994·European Journal of Immunology·A W Taylor-RobinsonR S Phillips
Sep 1, 1993·The American Review of Respiratory Disease·F P NijkampG Folkerts
Feb 21, 1997·The Journal of Biological Chemistry·E P GarveyR G Knowles
Feb 1, 1997·The American Journal of Physiology·H SchramekW Pfaller
Nov 14, 1997·British Journal of Pharmacology·S L UnderwoodJ A Karlsson
Jan 7, 1998·The Journal of Infectious Diseases·C C CorriveauR L Danner
Feb 6, 1999·Journal of Biomedical Science·D WangH I Chen
Oct 13, 2000·American Journal of Respiratory and Critical Care Medicine·W SteudelW M Zapol
Feb 28, 2001·Anesthesia and Analgesia·V StrunkG F Rich
Mar 17, 2001·Clinical and Experimental Pharmacology & Physiology·R P LeeH I Chen
Apr 20, 2001·British Journal of Pharmacology·E B HaddadM G Belvisi
Apr 24, 2001·American Journal of Respiratory and Critical Care Medicine·S WeickerS Mehta
Apr 20, 2002·Current Opinion in Allergy and Clinical Immunology·S A Kharitonov, P J Barnes
Jul 10, 2002·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Sergei A Kharitonov, Peter J Barnes
Sep 24, 2002·Pharmacology & Therapeutics·B J Nevin, K J Broadley
Sep 28, 2002·Critical Care Medicine·Thomas P ShanleyPeter A Ward
Feb 27, 2003·The Journal of Pharmacology and Experimental Therapeutics·Mark A BirrellMaria G Belvisi
May 29, 2003·Critical Care Medicine·Tiziana BachettiRoberto Ferrari
Sep 16, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Tatsuya OkamotoAlbert van der Vliet
Nov 25, 2003·The American Journal of Pathology·Cecilia L SpeyerPeter A Ward
Citations
May 1, 2010·Archives of Oral Biology·Ana NemecUros Skaleric
Apr 5, 2005·American Journal of Respiratory and Critical Care Medicine·Mark A BirrellMaria G Belvisi
May 20, 2006·American Journal of Respiratory Cell and Molecular Biology·Carla M PradoIolanda F L C Tibério
Aug 5, 2009·Respiratory Research·Sissie WongMark A Birrell
Jan 1, 2011·ISRN Allergy·Carla M PradoIolanda F L C Tibério
Feb 18, 2011·The Journal of Surgical Research·Ching-Wen KungMao-Hsiung Yen
Jan 30, 2010·Fish & Shellfish Immunology·Mari InadaToshiaki Itami
May 7, 2009·Oral Microbiology and Immunology·A NemecU Skaleric
Jan 6, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Vladimir Murashov
Jan 28, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Sachin KumarMadhu Dikshit
Feb 24, 2016·Water and Environment Journal : the Journal·Vedastus W Makene, Edmund J Pool
May 22, 2009·Acta Veterinaria Hungarica·Ana NemecMilan Petelin
Feb 11, 2015·International Immunopharmacology·Na-Rae ShinKyung-Seop Ahn
Jan 1, 2009·Sensors·Chuji Wang, Peeyush Sahay
Sep 20, 2012·Journal of Breath Research·Nhât-Nam Le-DongAnh Tuan Dinh-Xuan
Jul 31, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Magdalena StroblDiana Bonderman
Jul 25, 2006·The Journal of Pharmacology and Experimental Therapeutics·Kerryn McCluskieG Roger Thomas
May 13, 2006·The Journal of Pharmacology and Experimental Therapeutics·Mark A BirrellMaria G Belvisi
Mar 1, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M A BirrellM G Belvisi
Dec 13, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennifer L Head WheelerB Paige Lawrence
Sep 4, 2007·The Journal of Biological Chemistry·Mark A BirrellMaria G Belvisi
Jun 30, 2019·Oxidative Medicine and Cellular Longevity·Bartosz ProniewskiStefan Chlopicki
Jul 21, 2009·Biochemical Society Transactions·Christian HesslingerClemens Braun
Jun 20, 2020·International Journal of Clinical Practice·Hélida C Aquino-SantosRodolfo P Vieira
May 2, 2009·Journal of Applied Physiology·Tanveer AhmadAnurag Agrawal
Dec 7, 2019·Cellular Signalling·R Scott DuncanPeter Koulen
Feb 23, 2021·Medical Devices & Sensors·Fereshteh VajhadinAbass Amini