Nitric oxide binding to the heme of neuronal nitric-oxide synthase links its activity to changes in oxygen tension.

The Journal of Biological Chemistry
H M Abu-SoudD J Stuehr

Abstract

Neuronal nitric-oxide synthase (NOS-1) is a hemeprotein that generates NO and citrulline from L-arginine, O2, and NADPH. During catalysis, a majority of NOS-1 binds self-generated NO and converts to a ferrous-NO complex, which causes it to operate at a fraction of its maximum possible activity during the steady state (Abu-Soud, H. M., Wang, J., Rousseau, D. L., Fukuto, J., Ignarro, L. J., and Stuehr, D. J. (1995) J. Biol. Chem. 270, 22997-23006). To examine how NO complex formation affects the O2 response of NOS-1, we measured rates of NO synthesis and NADPH oxidation versus O2 concentration in the presence and absence of L-arginine. In the absence of L-arginine, NOS-1 catalyzed simple O2 reduction, and its heme iron displayed a typical affinity for O2 (estimated KmO2 </= 40 microM, saturation at approximately 100 microM). In the presence of L-arginine, the rates of NO synthesis and NADPH oxidation were proportional to the O2 concentration over a much broader range (estimated KmO2 approximately 400 microM, saturation at approximately 800 microM), indicating that ferrous-NO complex formation altered the O2 response of NOS-1. Stopped-flow experiments revealed that the rate of ferrous-NO complex formation was relatively independen...Continue Reading

References

Jan 1, 1977·Advances in Experimental Medicine and Biology·R W Estabrook, J Werringloer
Jun 1, 1991·The American Journal of Physiology·J M VanderkooiI A Silver
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R G KnowlesS Moncada
Jan 1, 1994·Progress in Neurobiology·S R Vincent
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H M Abu-Soud, D J Stuehr
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·J WangD J Stuehr
Jan 1, 1995·Annual Review of Physiology·J Garthwaite, C L Boulton
Jan 1, 1995·Annual Review of Physiology·O W Griffith, D J Stuehr
Jul 14, 1995·Journal of the Autonomic Nervous System·P A GrimesS Lahiri
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T M DawsonS H Snyder
Oct 15, 1993·Brain Research·N R PrabhakarM A Haxhiu
Sep 23, 1994·Cell·H H Schmidt, U Walter
Sep 9, 1993·Nature·C YoungsonE Cutz
Jan 1, 1993·Archives of Biochemistry and Biophysics·D A WinkR W Nims

❮ Previous
Next ❯

Citations

Dec 31, 2002·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Barbara E TaylorJohn E Remmers
Feb 22, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Nanduri R Prabhakar, Gregg L Semenza
May 24, 2003·Biochemical and Biophysical Research Communications·Silvia AlvarezAlberto Boveris
Aug 7, 2002·European Journal of Pharmacology·Manizheh KaramiHedayat Sahraei
Sep 1, 2000·Respiration Physiology·B E Taylor, K Lukowiak
Nov 24, 2001·Respiration Physiology·M Erecińska, I A Silver
Jun 29, 1999·Respiration Physiology·N R Prabhakar
Apr 11, 2001·Journal of Inorganic Biochemistry·S Adak, D J Stuehr
Aug 26, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·G U Dachs, G M Tozer
Oct 16, 2003·Nitric Oxide : Biology and Chemistry·Mikulas ChavkoRichard M McCarron
Sep 6, 2000·The Biochemical Journal·C C McCormickM Calero
Jul 21, 2001·The Biochemical Journal·W K AldertonR G Knowles
Oct 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·Anusha MishraEric A Newman
Oct 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Albert K Y TsuiGregory M T Hare
Mar 16, 2000·The Laryngoscope·J S HaightN Zamel
Jan 14, 2011·Plastic and Reconstructive Surgery·Stephen R Thom
Sep 5, 2009·Neuroreport·Yusuke OhgamiRaymond M Quock
Jan 13, 2005·Genome Génome / Conseil National De Recherches Canada·Tim DumonceauxRaju Datla
Mar 21, 1998·The Journal of Clinical Investigation·R A DweikS C Erzurum
Nov 9, 2005·The Journal of Clinical Investigation·Michael E WardPhilip A Marsden
Mar 15, 2005·The Journal of Clinical Investigation·Joshua M Hare, Jonathan S Stamler
Aug 22, 2002·Redox Report : Communications in Free Radical Research·Timothy M MillarCliff R Stevens
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Semira GalijasevicHusam M Abu-Soud
Oct 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Gloria A BenavidesDavid W Kraus
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Mohammad Mahfuzul HaqueDennis J Stuehr
Nov 24, 2001·Nature·C M BeallS C Erzurum
Apr 29, 1999·European Journal of Cell Biology·M Wilken, B Huchzermeyer
Oct 1, 2011·Free Radical Biology & Medicine·Mary A RobinsonCynthia M Otto
Apr 3, 2010·Archives of Oral Biology·Jelena RoganovićDragica Stojić
Apr 18, 2009·Respiratory Physiology & Neurobiology·Erica M Granjeiro, Benedito H Machado
May 29, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Maria ErecinskaIan A Silver
Apr 29, 2008·Free Radical Biology & Medicine·Douglas D ThomasDavid A Wink
Aug 20, 2009·The FEBS Journal·Jesús TejeroDennis J Stuehr
Jun 8, 2013·Dermatologic Therapy·Lee J Goldstein
Apr 16, 2009·Phytotherapy Research : PTR·Priya VenkatakrishnanR Timothy Miller
Jun 29, 2004·Microvascular Research·Kathleen A Lamkin-KennardDov Jaron
May 24, 2006·Free Radical Biology & Medicine·Erica M RichardsGary Fiskum
May 24, 2015·The Journal of Physiological Sciences : JPS·Philip LewisKen D O'Halloran
May 1, 1997·Neuron·J S StamlerN J Sucher

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.