PMID: 9523571Apr 2, 1998Paper

Nitric oxide causes glutamate release from brain synaptosomes

Journal of Neurochemistry
K S McNaught, G C Brown

Abstract

We determined the ability of pathological levels of nitric oxide (NO) to cause glutamate release from isolated rat brain nerve terminals using a fluorometric assay. It was found that NO (0.7 and 2 microM) produced (4 and 10 nmol/mg of synaptosomal protein) Ca2+-independent glutamate release from synaptosomes (after 1 min of exposure). Spermine/NO complex (spermine NONOate; a slow NO donor) and potassium cyanide (an inhibitor of cytochrome oxidase) also caused Ca2+-independent glutamate release. Preincubation of synaptosomes with 5 microM 1H-[1,2,4]oxadiazole[4,3-a]quinoxalin-1-one (an inhibitor of soluble guanylyl cyclase) had no effect on NO-induced Ca2+-independent glutamate release. Ca2+-independent glutamate release produced by NO was greater in a low-oxygen medium. NO, spermine NONOate, and potassium cyanide inhibited synaptosomal respiration with a similar order of potency with respect to their ability to cause glutamate release. Because NO has been shown previously to inhibit reversibly cytochrome oxidase in competition with oxygen, our findings in this study suggest that NO (and cyanide) causes glutamate release following inhibition of mitochondrial respiration at the level of cytochrome oxidase. Thus, elevated NO produ...Continue Reading

Citations

Sep 15, 2000·Journal of Neurochemistry·A Bal-Price, G C Brown
May 22, 2001·Experimental Biology and Medicine·K K ParkK F Soliman
Dec 18, 2001·Journal of Neuroscience Research·H YuS D Cederbaum
Nov 26, 2011·Clinical Reviews in Allergy & Immunology·Wesley H Brooks
Sep 30, 2015·The European Journal of Neuroscience·Ruben EggersJoost Verhaagen
Mar 6, 2002·The European Journal of Neuroscience·V C StewartS J R Heales
Oct 25, 2007·Biochemical Society Transactions·G C Brown
Sep 22, 2010·Journal of Basic and Clinical Physiology and Pharmacology·I StojanovićT Jevtović-Stoimenov
Aug 31, 2016·The Journal of Pharmacology and Experimental Therapeutics·Samar Rezq, Abdel A Abdel-Rahman
Sep 29, 2000·American Journal of Physiology. Heart and Circulatory Physiology·A BhardwajR C Koehler
Sep 10, 2004·American Journal of Physiology. Cell Physiology·Paul S BrookesShey-Shing Sheu
Feb 15, 2000·Annals of the New York Academy of Sciences·A Bal-PriceG C Brown
Mar 3, 2010·Molecular Neurobiology·Guy C Brown, Jonas J Neher
May 13, 1999·Biochimica Et Biophysica Acta·G C Brown
Jan 25, 2003·Free Radical Biology & Medicine·Victoria C Stewart, Simon J R Heales
Aug 20, 2021·Aging Cell·Eduardo J Fernandez-PerezLuis G Aguayo
Apr 30, 2020·Current Neuropharmacology·Devesh TewariSeyed M Nabavi

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