Nitric oxide complexes of inducible nitric oxide synthase: spectral characterization and effect on catalytic activity

Biochemistry
Amy R Hurshman, Michael A Marletta

Abstract

Nitric oxide synthase (NOS) catalyzes the oxidation of L-arginine to citrulline and nitric oxide (.NO). NOS is a hemoprotein containing a cytochrome P-450-type heme that has been shown to be involved in catalysis. It has been suggested that .NO is able to bind tightly to the heme of NOS and may in this way serve to regulate enzymatic activity. We report here the formation of both ferric and ferrous heme nitrosyl complexes with the inducible NOS from murine macrophages. The ferric nitrosyl complex is characterized by a Soret peak at 443 nm and two distinct peaks in the alpha/beta region at 549 and 585 nm. The ferrous nitrosyl complex has absorbance maxima at 436 and 566 nm. A transient spectral intermediate is observed under conditions of NOS turnover. This intermediate appears to be a mixture of ferric and ferrous nitrosyl complexes and is unstable in the presence of oxygen. Binding of L-arginine decreases the affinity of .NO for the ferric heme but does not appear to decrease the affinity of .NO for the ferrous heme. Addition of either oxyhemoglobin or methemoglobin to NOS assays results in a nearly 2-fold increase in enzymatic activity. This result is attributed to the ability of both forms of hemoglobin to decrease the conce...Continue Reading

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