Nitric oxide contributes to the development of a post-injury Th2 T-cell phenotype and immune dysfunction

Journal of Cellular Physiology
Tanjanika DanielMartin G Schwacha

Abstract

Severe injury induces immune dysfunction resulting in increased susceptibility to opportunistic infections. Previous studies from our laboratory have demonstrated that post-burn immunosuppression is mediated by nitric oxide (NO) due to the increased expression of macrophage inducible nitric oxide synthase (iNOS). In contrast, others suggest that injury causes a phenotypic imbalance in the regulation of Th1- and Th2 immune responses. It is unclear whether or not these apparently divergent mediators of immunosuppression are interrelated. To study this, C57BL/6 mice were subjected to major burn injury and splenocytes were isolated 7 days later and stimulated with antiCD3. Burn injury induced NO-mediated suppression of proliferative responses that was reversed in the presence of the NOS inhibitor L-monomethyl-L-arginine and subsequently mimicked by the addition of the NO donor, S-nitroso-N-acetyl-penicillamine (SNAP). SNAP also dose-dependently suppressed IFN-gamma and IL-2 (Th1), but not IL-4 and IL-10 (Th2) production. Delaying the addition of SNAP to the cultures by 24 h prevented the suppression of IFN-gamma production. The Th2 shift in immune phenotype was independent of cGMP and apoptosis. The addition of SNAP to cell culture...Continue Reading

References

Jan 1, 1992·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·T BambergerI Florentin
Nov 1, 1995·The Journal of Trauma·R L GamelliZ Radisavljevic
Nov 24, 1995·Science·Z XiaM E Greenberg
Nov 1, 1995·Archives of Surgery·J T DiPiroA R Mansberger
Jun 1, 1993·The Journal of Trauma·W K BeckerB A Pruitt
Apr 1, 1996·The Journal of Trauma·R M SherryJ T DiPiro
Jan 1, 1997·Annual Review of Immunology·H NakamuraJ Yodoi
Nov 5, 1997·Annals of Plastic Surgery·T Sozumi
Oct 20, 1998·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·S M PaulsenL B Nanney
Apr 3, 1999·Shock·J A LedererJ A Mannick
Aug 24, 1999·European Journal of Immunology·W NiedbalaF Y Liew
Feb 19, 2000·Current Opinion in Immunology·C BogdanA Diefenbach
Mar 17, 2000·Immunological Reviews·C BogdanA Diefenbach
Feb 17, 2001·American Journal of Respiratory and Critical Care Medicine·R S Munford, J Pugin
Mar 20, 2001·American Journal of Respiratory and Critical Care Medicine·K SoejimaL Varig
Aug 23, 2001·International Immunopharmacology·H Schindler, C Bogdan
Sep 8, 2001·American Journal of Physiology. Cell Physiology·M G SchwachaI H Chaudry
Sep 18, 2002·Journal of Molecular and Cellular Cardiology·Takamichi UchiyamaYonosuke Kobayashi
Nov 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·Wanda NiedbalaFoo Y Liew
Jan 10, 2003·The New England Journal of Medicine·Richard S Hotchkiss, Irene E Karl
Jan 25, 2003·Burns : Journal of the International Society for Burn Injuries·Martin G Schwacha
Apr 22, 2003·Journal of Cellular Physiology·M A Santana, Y Rosenstein
Jul 18, 2003·Cell Death and Differentiation·Bernhard Brüne
Jul 23, 2003·Journal of the American Society of Nephrology : JASN·Josef PfeilschifterAndrea Huwiler
Oct 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zhijun GuoJames A Lederer

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Citations

Oct 27, 2010·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Miriam Ojeda OjedaManuel de Jesús Araña Rosaínz
Dec 28, 2006·Apoptosis : an International Journal on Programmed Cell Death·G GravanteG Sconocchia
Oct 2, 2008·Biological & Pharmaceutical Bulletin·Keizo KohnoShigeharu Fukuda
Jan 21, 2010·Cellular and Molecular Life Sciences : CMLS·Julie PatenaudeJacques Bernier
Jan 8, 2016·Burns : Journal of the International Society for Burn Injuries·Yanbin GaoGuifang Wang
Sep 29, 2009·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Jun FanLiang Ma
Dec 1, 2007·Injury·Sven K Tschoeke, Wolfgang Ertel
Oct 26, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·C DuA M Jevnikar
Mar 11, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Thiagavathi S Ramyaa LakshmiMary Babu
Jun 9, 2016·Burns : Journal of the International Society for Burn Injuries·Julia L M DunnMark H Schoenfisch
Apr 4, 2017·International Journal of Molecular Sciences·Albert KimJohn Vandervord
May 19, 2018·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Min LianXiong Ma
Feb 19, 2015·Shock·Xiaoling LiMashkoor A Choudhry
Nov 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Agnes KonczGyorgy Nagy

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