Nitric oxide decreases cytokine-induced endothelial activation. Nitric oxide selectively reduces endothelial expression of adhesion molecules and proinflammatory cytokines

The Journal of Clinical Investigation
R De CaterinaJ K Liao

Abstract

To test the hypothesis that nitric oxide (NO) limits endothelial activation, we treated cytokine-stimulated human saphenous vein endothelial cells with several NO donors and assessed their effects on the inducible expression of vascular cell adhesion molecule-1 (VCAM-1). In a concentration-dependent manner, NO inhibited interleukin (IL)-1 alpha-stimulated VCAM-1 expression by 35-55% as determined by cell surface enzyme immunoassays and flow cytometry. This inhibition was paralleled by reduced monocyte adhesion to endothelial monolayers in nonstatic assays, was unaffected by cGMP analogues, and was quantitatively similar after stimulation by either IL-1 alpha, IL-1 beta, IL-4, tumor necrosis factor (TNF alpha), or bacterial lipopolysaccharide. NO also decreased the endothelial expression of other leukocyte adhesion molecules (E-selectin and to a lesser extent, intercellular adhesion molecule-1) and secretable cytokines (IL-6 and IL-8). Inhibition of endogenous NO production by L-N-monomethyl-arginine also induced the expression of VCAM-1, but did not augment cytokine-induced VCAM-1 expression. Nuclear run-on assays, transfection studies using various VCAM-1 promoter reporter gene constructs, and electrophoretic mobility shift as...Continue Reading

References

Dec 1, 1992·The Journal of Experimental Medicine·A S NeishT Collins
Nov 1, 1992·British Journal of Pharmacology·M W RadomskiS Moncada
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J S StamlerJ Loscalzo
Jan 1, 1992·Free Radical Research Communications·R SchreckP A Baeuerle
Sep 1, 1992·The Journal of Clinical Investigation·J P CookeM E Billingham
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·P KubesD N Granger
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M W RadomskiS Moncada
Mar 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·P M BathJ F Martin
Apr 1, 1991·American Journal of Respiratory Cell and Molecular Biology·F J WaltherH J Forman
Jan 1, 1991·Annual Review of Immunology·J J OppenheimK Matsushima
Aug 2, 1990·Nature·T A Springer
Jan 1, 1990·Annual Review of Pharmacology and Toxicology·L J Ignarro
Jan 1, 1990·Annual Review of Immunology·J Van Snick
May 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·S J WarnerP Libby
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M P BevilacquaM A Gimbrone
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Jan 6, 1995·The Journal of Biological Chemistry·J K LiaoS L Clark
Jul 1, 1993·Circulation Research·I KuroseD N Granger
May 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·J P Cooke, P S Tsao
Jan 1, 1993·Free Radical Research Communications·R E Huie, S Padmaja

❮ Previous
Next ❯

Citations

Dec 11, 2008·JAMA : the Journal of the American Medical Association·Gregory J Kato, Mark T Gladwin
Mar 3, 2011·JAMA : the Journal of the American Medical Association·Mark T GladwinUNKNOWN DeNOVO Investigators
Feb 24, 2000·The Journal of Pathology·H Li, U Förstermann
Sep 1, 1997·Journal of Cellular Physiology·T VolkW J Kox
Jun 16, 2001·Arthritis and Rheumatism·A VeihelmannF Krombach
Jul 21, 1998·Clinical Cardiology·U IkedaK Shimada
Aug 1, 1997·Basic Research in Cardiology·R J GuminaG J Gross
Jun 1, 1997·Metabolic Brain Disease·J E Merrill, S P Murphy
Apr 20, 2000·Indian Journal of Pediatrics·M S KannanD E Johnson
Apr 13, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Aleksandra Bielawska-PohlSalem Chouaib
Jun 1, 2011·Intensive Care Medicine·M RevermannR P Brandes
Aug 2, 2011·Seminars in Immunopathology·Hideru Obinata, Timothy Hla
May 24, 2005·Zeitschrift für Rheumatologie·F MoritzH Häntzschel
Apr 25, 2006·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Tsutomu SakaiKenji Kitahara
Nov 13, 2008·European Journal of Applied Physiology·R V VinceL A Madden
Feb 15, 2011·Pflügers Archiv : European journal of physiology·Katrin KlicheHans Oberleithner
Nov 16, 2011·Pflügers Archiv : European journal of physiology·Richard S T LeungDaniel Stevens
Oct 20, 2009·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Giuseppe BomboiMassimo Volpe
Feb 27, 2008·Annals of Biomedical Engineering·Julie Y JiScott L Diamond
Jun 6, 2009·Journal of Inherited Metabolic Disease·J M Politei
May 1, 2007·Cardiovascular Drugs and Therapy·Vineet ChopraErdal Cavusoglu
Jan 16, 2007·Journal of Thrombosis and Thrombolysis·Luci Maria SantAna DusseBashir A Lwaleed
Mar 14, 2008·Journal of Thrombosis and Thrombolysis·Francesca MarzoFelicita Andreotti
Sep 19, 2012·Purinergic Signalling·Suellen D'Arc Dos Santos OliveiraClaudia Lucia Martins Silva
Dec 21, 2000·Current Atherosclerosis Reports·M R AdamsA P Selwyn
Apr 5, 2001·Current Atherosclerosis Reports·J P Cooke, R K Oka
Aug 7, 2001·Current Atherosclerosis Reports·J C Plana, P H Jones
Dec 21, 2000·Current Atherosclerosis Reports·M MaytinJ Loscalzo
Mar 20, 2003·Current Diabetes Reports·Patrick H McNulty
May 24, 2003·Current Diabetes Reports·Pierre Theuma, Vivian A Fonseca
May 2, 2003·Current Hypertension Reports·Stefan John, Roland E Schmieder
Aug 3, 2005·Current Hypertension Reports·Howard S Weintraub
Nov 15, 2005·Current Treatment Options in Cardiovascular Medicine·Sajoscha Sorrentino, Ulf Landmesser
Sep 25, 2008·Cardiovascular Toxicology·Leena PradhanKrishna C Agrawal
Aug 1, 1996·Cytokine & Growth Factor Reviews·P Beauparlant, J Hiscott

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