Nitric oxide decreases the stability of DMPO spin adducts

Nitric Oxide : Biology and Chemistry
Krzysztof J ReszkaBradley E Britigan

Abstract

The effect nitric oxide (NO*) on the stability of 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) adducts has been investigated using EPR spectroscopy. We report that the DMPO/HO* adduct, generated by porcine pulmonary artery endothelial cells in the presence of H2O2 and DMPO, or by a Fenton system (Fe(II)+H2O2) is degraded in the presence of the NO*-donor, 2-(N,N-diethylamino)-diazenolate-2-oxide (DEANO) or by bolus addition of an aqueous solution of NO*. A similar effect of DEANO was observed on other DMPO adducts, such as DMPO/*CH3 and DMPO/*CH(CH3)OH, generated in cell-free systems. Measurements of the loss of DMPO/HO* in the presence of DEANO in aerated and oxygen-free buffers showed that in both of these settings the process obeys first-order kinetics and proceeds with similar efficacy. This indicates that direct interaction of the nitroxide with NO*, rather than with NO2* (formed from NO* and O2 in aerated media), is responsible for destruction of the spin adduct. These results suggest that the presence of NO* may substantially affect the quantitative determination of DMPO adducts. We also show that NO2* radicals, generated by a myeloperoxidase/H2O2/nitrite system, also degrade DMPO/HO*. Because DMPO is frequently used to study ...Continue Reading

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Citations

Oct 12, 2013·Inorganic Chemistry·Asli CangönülMaurice van Gastel
Jul 20, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·David ContrerasWolfgang Kaim
Jul 30, 2010·Ultrasonics Sonochemistry·Sadie DeanJesús Iniesta
Feb 19, 2008·Nitric Oxide : Biology and Chemistry·Karuthapillai GovindarajuDavid H Eidelman
Aug 7, 2009·Journal of the American Chemical Society·Chunmao He, Markus Knipp
Mar 16, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jana KianičkaJuraj Filo

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