Nitric oxide-dependent long-term potentiation revealed by real-time imaging of nitric oxide production and neuronal excitation in the dorsal horn of rat spinal cord slices

The European Journal of Neuroscience
Hiroshi IkedaKazuyuki Murase

Abstract

Nitric oxide (NO) is thought to be involved in the central mechanism of hyperalgesia and allodynia at the spinal level. Recently, we reported that NO played an important role in the induction of long-term potentiation (LTP) of synaptic strength in spinal dorsal horn, which is believed to underlie hyperalgesia and allodynia. In this study, to elucidate the relationship of NO to LTP in spinal dorsal horn, we measured the spatiotemporal distribution of NO signal with the NO-sensitive dye, DAR-4M, and neuronal excitation with the voltage-sensitive dye, RH482, in rat spinal cord slices, elicited by dorsal root stimulation. In superficial dorsal horn, neuronal excitation evoked by C fiber-activating dorsal root stimulation was potentiated for more than 2 h after low-frequency conditioning stimulation (LFS, 240 pulses at 2 Hz for 2 min). In the same slices that exhibited LTP, NO was produced and distributed in the superficial dorsal horn during the delivery of LFS, and the amplitude of LTP and amount of NO production showed close correlation from slice to slice. LTP and production of NO were inhibited in the presence of the NO synthase inhibitors and an inhibitor of heme oxygenase, the synthetic enzyme for carbon monoxide (CO). These ...Continue Reading

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Citations

Aug 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yun GuanXinzhong Dong
Feb 15, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Laszlo KölesPeter Illes
Mar 30, 2011·Molecular Pain·Ruth RuscheweyhJürgen Sandkühler
Jun 16, 2009·Free Radical Biology & Medicine·Hao HongWeibo Cai
Sep 13, 2015·Nitric Oxide : Biology and Chemistry·Mayank GautamSubrata Basu Ray
Nov 30, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Gin-Den ChenTzer-Bin Lin

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