Nitric oxide-dependent parasympathetic signaling is due to activation of constitutive endothelial (type III) nitric oxide synthase in cardiac myocytes.

The Journal of Biological Chemistry
J L BalligandT Michel

Abstract

Nitric oxide synthase (NOS) isoforms are discovered in an increasing variety of cell types with different roles in signaling. The inducible NOS (i.e. iNOS or NOS II) is expressed in cardiac myocytes in response to specific cytokines. Independent of iNOS induction, however, receptor-dependent signaling is modulated by a constitutive nitric oxide (NO) synthase isoform in these cells (Balligand, J. L., Kelly, R.A., Marsden, P.A., Smith, T. W., and Michel, T. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 347-351). We now show that cardiac myocytes constitutively express the endothelial isoform of NO synthase (ecNOS or NOS III). Transcripts for NOS III were detected by Northern blot in myocyte extracts using as a probe a polymerase chain reaction-generated cDNA amplified with isoform and species-specific primers. In subcellular fractionation experiments, a calcium-sensitive NO synthase activity was present primarily in the particulate fraction, coinciding with the distribution of NOS III analyzed by protein immunoblotting. The localization of NOS III within cardiac myocytes was further demonstrated by immunohistochemistry. The functional role of NOS III was explored by analyzing the effects of NOS inhibitors on single myocyte L-type calc...Continue Reading

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S LamasT Michel
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M C MagliE Boncinelli
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J S PollockF Murad
Jan 1, 1990·Annual Review of Physiology·W Trautwein, J Hescheler
Aug 1, 1988·The Journal of General Physiology·R Horn, A Marty
Jan 1, 1988·Progress in Biophysics and Molecular Biology·H C Hartzell
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·J L DinermanS H Snyder
Sep 23, 1994·Cell·C Nathan, Q W Xie
Dec 8, 1994·Nature·L KobzikJ S Stamler
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J L BalligandT Michel
Apr 15, 1994·The Journal of Physiology·X HanW R Giles
Jul 3, 1993·Lancet·A Coates, J Forbes

❮ Previous
Next ❯

Citations

Jan 1, 1996·Basic Research in Cardiology·L Hove-MadsenR Fischmeister
May 29, 2012·Pediatric Cardiology·Daniela Malan, Bernd K Fleischmann
Mar 29, 2005·Basic Research in Cardiology·J Vinten-JohansenF Kerendi
Jun 20, 2012·Current Heart Failure Reports·Matthew S Alkaitis, Mark J Crabtree
Mar 31, 2004·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·A R WhiteR J Walker
Dec 3, 2003·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Kazim Husain
Jun 14, 2005·Pharmacology & Therapeutics·R SchulzJ-L Balligand
Feb 12, 2004·Journal of Molecular and Cellular Cardiology·Ahlke HeydemannElizabeth M McNally
May 30, 1996·The American Journal of Cardiology·R A Kelly, T W Smith
Jan 4, 2001·European Journal of Pharmacology·A NahavandiM R Abdolhoseini
Feb 13, 1999·European Journal of Pharmacology·E J WhalenS J Lewis
Jun 24, 2003·Journal of Molecular and Cellular Cardiology·Joshua M Hare
Nov 1, 2003·Thrombosis Research·Maria MadajkaTadeusz Malinski
Sep 29, 1999·Pharmacology & Therapeutics·L Piacentini, J S Karliner
Apr 13, 2000·Pharmacology & Therapeutics·A M Shah, P A MacCarthy
Nov 10, 1998·Journal of the Autonomic Nervous System·C E SearsD J Paterson
May 26, 1999·Infectious Disease Clinics of North America·S Symeonides, R A Balk
May 20, 1998·Nutrition·B Alexander
Jan 20, 1998·Trends in Biochemical Sciences·B Mayer, B Hemmens
Jul 16, 2003·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Kazim Husain
Aug 1, 1996·Trends in Cardiovascular Medicine·P Brecher
Jan 1, 1997·Trends in Cardiovascular Medicine·K Sase, T Michel
Jul 1, 1997·Trends in Cardiovascular Medicine·H DrexlerC Holubarsch
Sep 12, 2002·Nitric Oxide : Biology and Chemistry·Huige LiUlrich Förstermann
Mar 20, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·M B ReidJ S Stamler
Feb 12, 1998·British Journal of Pharmacology·G KojdaE Noack
Jan 12, 2002·British Journal of Pharmacology·Yinke YangJames P Morgan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.