PMID: 8978714Jan 1, 1997Paper

Nitric oxide disrupts Ca2+ homeostasis in hippocampal neurons

Journal of Neurochemistry
J R BrorsonH Zhang

Abstract

Nitric oxide has been recognized in recent years as an important mediator of neuronal toxicity, which in many cases involves alterations of the cytoplasmic Ca2+ concentration ([Ca2+]i). In [Ca2+]i fluorimetric experiments on cultured hippocampal neurons, the nitric oxide-releasing agent S-nitrosocysteine produced a delayed rise in [Ca2+]i over a 20-min exposure, which was accompanied by a progressive slowing of the kinetics of recovery from depolarization-induced [Ca2+]i transients. These effects were blocked by oxyhemoglobin and by superoxide dismutase, confirming nitric oxide as the responsible agent, and suggesting that they involved peroxynitrite formation. Similar alterations of [Ca2+]i homeostasis were produced by the mitochondrial ATP synthase inhibitor oligomycin, and when an ATP-regenerating system was supplied via the patch pipette in combined whole-cell patch-clamp-[Ca2+]i fluorimetry experiments, S-nitrosocysteine had no effect on the resting [Ca2+]i or on the recovery kinetics of [Ca2+]i transients induced by direct depolarization. We conclude that prolonged exposure to nitric oxide disrupts [Ca2+]i homeostasis in hippocampal neurons by impairing Ca2+ removal from the cytoplasm, possibly as a result of ATP depletio...Continue Reading

Citations

Jun 15, 2006·Antioxidants & Redox Signaling·Juan P Bolaños, Angeles Almeida
Aug 22, 2008·Antioxidants & Redox Signaling·Andrew B Knott, Ella Bossy-Wetzel
Sep 13, 2001·The European Journal of Neuroscience·W VandenbergheJ R Brorson
Jul 12, 2005·Cardiovascular Drug Reviews·Mercedes VillarroyaAntonio G García
Jun 30, 2006·Journal of Neurochemistry·Salvador Moncada, Juan P Bolaños
Mar 25, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S Jill JamesDavid W Gaylor
Jan 5, 2000·Physiological Reviews·D G Nicholls, S L Budd
Jun 30, 2000·Biochemical and Biophysical Research Communications·E MaltepeM C Simon
May 2, 2002·Clinical Biochemistry·Ludmila ZylinskaMiroslaw Soszynski

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