Abstract
The present study tested the hypothesis that acute increases in nitric oxide (NO) exert substantial influences on gene transcription in endothelial cells (ECs) via guanylyl cyclase (GC). Human umbilical veins ECs (HUVECs) were exposed to 0.1, 1, and 10 mM of sodium nitroprusside (SNP) for 4 h and to 1 mM SNP or 250 microM of (Z)-1[N-(2-aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate (DETA-NONOate) for 2, 4, 8, and 24 h. Also, cells were exposed to DETA-NONOate in the presence and absence of the GC inhibitor 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ; 10 microM) for 4 h. RNA was isolated, reverse transcribed, Cy3 and Cy5 labeled, and analyzed using cDNA microarrays. Increasing doses of SNP predominantly depressed gene expression in HUVECs. Gene function was related to growth, adhesion, and cell structure. DETA-NONOate evoked a wave of expression changes (maximum at 4 h), with a remarkable downregulation of the transcription factors MSX1, RELB, and Egr-1. Both SNP- and DETA-NONOate-induced gene expression had faded after 24 h, despite continued elevation of cGMP in the medium. Coadministration of ODQ decreased many, but not all, of the transcriptional responses to DETA-NONOate. NO pronouncedly depressed EC gen...Continue Reading
References
Nov 1, 1973·The Journal of Clinical Investigation·E A JaffeC R Minick
Mar 1, 1967·Clinical Pharmacology and Therapeutics·J L Schelling, L Lasagna
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Jul 1, 1995·The Journal of Clinical Investigation·R De CaterinaJ K Liao
Jan 1, 1995·The Journal of Clinical Investigation·T MünzelD G Harrison
May 30, 1996·The American Journal of Cardiology·T MünzelD G Harrison
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·M A MoroS Moncada
Aug 1, 1996·Cytokine & Growth Factor Reviews·P Beauparlant, J Hiscott
Mar 4, 1997·Circulation·S M YuC C Lin
Aug 15, 1997·The Journal of Clinical Investigation·G FilippovK D Bloch
Feb 7, 1998·Hypertension·T IchikiA Takeshita
Oct 14, 1998·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·V Lindner
Mar 6, 1999·The Journal of Biological Chemistry·L RössigS Dimmeler
Apr 9, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·X BaoJ A Frangos
Jun 25, 1999·Atherosclerosis·R HellerU Till
Aug 7, 1999·Circulation Research·J J ChiuD L Wang
Dec 10, 1999·The Journal of Experimental Medicine·A HmadchaE Pintado
Jan 5, 2000·Kidney International·H D RupprechtG Hofer
Jan 8, 2000·Microvascular Research·R BerkelsW Klaus
Feb 15, 2000·European Journal of Biochemistry·M SwiatkowskaC S Cierniewski
Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·T C BellamyJ Garthwaite
Apr 20, 2000·European Journal of Pharmacology·O KosonenE Moilanen
Jul 25, 2000·Hypertension·M SataY Hirata
Nov 14, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·R De MartinJ A Schmid
Feb 13, 2001·Trends in Cell Biology·C Bogdan
Jun 8, 2001·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Martin BraddockCallum J. Campbell
Jul 18, 2001·The Journal of Biological Chemistry·K Y KimM J Nam
Aug 21, 2001·Pflügers Archiv : European journal of physiology·J PfeilschifterK F Beck
Sep 27, 2001·Cell·J S StamlerF C Fang
Oct 18, 2001·The Journal of Cell Biology·F MullershausenA Friebe
Dec 1, 2001·Nature Genetics·A BrazmaM Vingron
Jun 11, 2002·American Journal of Physiology. Cell Physiology·Faton H AganiJoseph LaManna
Aug 24, 2002·Clinical Science·Mamoru OhkitaYasuo Matsumura
Aug 30, 2002·Biochemical and Biophysical Research Communications·Hideo KimuraHiroyasu Esumi
Sep 28, 2002·BMC Biochemistry·Rut Ferrero, Magdalena Torres
Dec 3, 2002·The American Journal of Cardiology·Dominik Behrendt, Peter Ganz
Jan 25, 2003·Annals of the New York Academy of Sciences·Aernout LuttunPeter Carmeliet
Apr 18, 2003·Physiological Genomics·Denise C PolacekPeter F Davies
May 30, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sarah E DuffShant Kumar
Citations
Aug 6, 2005·Bioinformatics·Jianhua HuKevin R Coombes
Mar 11, 2011·American Journal of Physiology. Cell Physiology·James A StewartPamela A Lucchesi
Oct 28, 2006·Radiation Research·Gaëtan GruelDiana Tronik-Le Roux
Jun 6, 2014·Toxins·Andréa E M StinghenAgnès Boullier
Mar 19, 2011·Cytokine·Jakub SiednienkoWojciech A Gorczyca
Nov 17, 2009·Life Sciences·Simon W Rabkin
May 26, 2009·Pharmacology & Therapeutics·Barbara IlliCarlo Gaetano
Dec 2, 2006·Stem Cells·Bogdan A DanalacheMarek Jankowski
Aug 19, 2006·Stem Cells·Ruxandra CovacuLou Brundin
Mar 1, 2006·Nitric Oxide : Biology and Chemistry·Thomas WaldowKlaus Matschke
Oct 30, 2012·Developmental Biology·Carmine GentileChristopher J Drake
Nov 18, 2008·Molecular Immunology·Mary Y K LeePaul M Vanhoutte
Nov 20, 2014·PloS One·Laura LabontéCarolyn J Baglole
Mar 8, 2006·Hypertension·Helena ChonBranko Braam
Feb 9, 2005·Hypertension·Branko BraamHein Koomans
Dec 14, 2005·Hypertension·Lonneke M BeversJaap A Joles
Oct 19, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Mary Y K LeePaul M Vanhoutte
Feb 26, 2016·PloS One·Laura E LabontéCarolyn J Baglole
May 11, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chanachai SaeleeTewin Tencomnao
Jul 26, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Eva H C TangPaul M Vanhoutte
Oct 19, 2006·Physiological Genomics·Sebastiaan WesselingBranko Braam
Mar 13, 2009·Organogenesis·Maarten P KoenersJaap A Joles
Feb 4, 2006·Blood·Rob J DekkerAnton J G Horrevoets
Feb 25, 2011·Cardiovascular Research·Barbara IlliCarlo Gaetano
Mar 5, 2013·Molecular and Cellular Biochemistry·Cesar A MeschiariJose E Tanus-Santos
Dec 22, 2020·SLAS Technology·Valeria Graceffa
Oct 12, 2010·Journal of Proteome Research·Yi-Ju ChenYu-Ju Chen