Nitric oxide from neuronal NOS plays critical role in cerebral capillary flow response to hypoxia

The American Journal of Physiology
A G HudetzJ P Kampine

Abstract

We investigated, using a direct, intravital microscopic technique, whether nitric oxide (NO) from neuronal nitric oxide synthase (nNOS) plays a role in the cerebral capillary flow response to acute hypoxia. Erythrocyte flow in subsurface capillaries of the frontoparietal cortex of adult Sprague-Dawley rats was visualized using epifluorescence videomicroscopy after fluorescent labeling of red blood cells (RBC) in tracer concentrations. The velocity of labeled RBC in individual capillaries was measured off-line using an image analysis system. Hypoxia was produced by lowering the inspired O2 concentration to 15% for 5 min in control animals and in those pretreated with the selective nNOS inhibitor 7-nitroindazole (7-NI; 20 mg/kg ip). In the control group, hypoxia increased RBC velocity by 34 +/- 8%. In the group treated with 7-NI, this response was reversed to a statistically significant 8 +/- 3% decrease. This paradoxical response to hypoxia after 7-NI was observed in nearly all capillaries. 7-NI itself decreased the baseline RBC velocity by 12 +/- 4%. The cerebral hyperemic response to hypoxia was also assessed with the laser Doppler flow (LDF) technique. In control animals, hypoxia produced a 33 +/- 6% increase in LDF, similar ...Continue Reading

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Citations

Jul 13, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·A P HarrisR C Koehler
May 18, 2001·American Journal of Physiology. Heart and Circulatory Physiology·G ZoccoliA M Walker
Mar 11, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Holly D Bauser-HeatonH Glenn Bohlen
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