Nitric oxide from rat liver sinusoidal endothelial cells induces apoptosis in IFN gamma-sensitized CC531s colon carcinoma cells

Journal of Hepatology
Katrien VekemansEddie Wisse

Abstract

Investigation of apoptosis is pivotal in searching for mechanisms that eliminate colon cancer cells getting trapped in liver sinusoids at the time of surgical removal of the primary tumor. This study focuses on nitric oxide (NO), Fas/FasL and the involvement of interferon-gamma (IFNgamma) in liver sinusoidal endothelial cells (LSECs) and in the colon carcinoma cell line CC531s. Apoptosis was quantified and visualized in vitro by specific DNA fragmentation, specific staining and electron microscopy. In vivo experiments were also conducted. In co-cultures of LSECs with CC531s, apoptosis of CC531s was observed only when they were pre-treated with IFNgamma, and was unaffected by blocking the Fas/FasL pathway. However, LSECs continuously produced NO, and apoptosis was inhibited by NO-inhibitors (NMMA and dexamethasone). When IFNgamma-sensitized CC531s were injected into rats, liver weight was lower, in contrast to control conditions where liver weight was higher. (i) LSECs induce apoptosis in IFNgamma-sensitized CC531s in vitro; (ii) LSECs express FasL; (iii) Fas on CC531s becomes active after IFNgamma-treatment; however, (iv) blocking the Fas/FasL pathway had no effect; (v) apoptosis was inhibited by NO-inhibitors; (vi) the immune ...Continue Reading

References

Jan 1, 1992·Cytometry·Z DarzynkiewiczF Traganos
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M W RadomskiS Moncada
May 15, 1984·International Journal of Cancer. Journal International Du Cancer·R L MarquetJ Jeekel
Nov 3, 1995·International Journal of Cancer. Journal International Du Cancer·M RochaV Umansky
Jan 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·F BraetE Wisse
Feb 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Z KanS Wallace
Jan 1, 1996·Toxicologic Pathology·E WisseA Vermoesen
Mar 19, 1997·Journal of the National Cancer Institute·K XieI J Fidler
Dec 24, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D VermijlenE Wisse
Nov 11, 1999·Cell Death and Differentiation·L Liu, J S Stamler
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·T WallerathU Förstermann
Mar 29, 2000·Journal of Hepatology·J R JonssonE E Powell
Apr 5, 2001·The Journal of Biological Chemistry·A CremestiR Kolesnick
Apr 9, 2002·Molecular and Cellular Biochemistry·Berit HansenBård Smedsrød
Feb 11, 2003·The Journal of Biological Chemistry·Jiang YangKenneth Walsh
Jul 29, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Olaf R F MookCornelis J F Van Noorden
Aug 5, 2003·Liver International : Official Journal of the International Association for the Study of the Liver·Katrien VekemansFilip Braet
Aug 5, 2003·The Journal of Pathology·Nasser A MohammedRaymond F T McMahon
Oct 28, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Katrien VekemansEddie Wisse
Mar 9, 2004·Micron : the International Research and Review Journal for Microscopy·Katrien VekemansFilip Braet
Feb 24, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shan-Shan ZhanNatalie J Torok
Jan 23, 2008·Journal of Cellular and Molecular Medicine·O R F MookC J F Van Noorden
Jun 26, 2010·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Jaione Simon-SantamariaKaren Kristine Sørensen

❮ Previous
Next ❯

Citations

Mar 11, 2011·World Journal of Gastroenterology : WJG·Kristina A JahnFilip Braet
Mar 9, 2007·Immunology·Naama Peshes-YalozGideon Berke
Aug 28, 2020·International Journal of Radiation Biology·João Victor da Silva CoutinhoSonia Alves Gouvea
Aug 30, 2005·World Journal of Gastroenterology : WJG·Katrien Vekemans, Filip Braet
Jan 30, 2019·Naunyn-Schmiedeberg's Archives of Pharmacology·Mohamed G EweesMohamed S Abdelbakky
Sep 29, 2020·Frontiers in Physiology·Alex L WilkinsonShishir Shetty

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis