PMID: 8607682Apr 1, 1996Paper

Nitric oxide in the oxygenator sweep gas reduces platelet activation during experimental perfusion

The Annals of Thoracic Surgery
Karin MellgrenH Wadenvik

Abstract

Hemorrhage is a major complication experienced in 10% to 35% of neonates treated with extracorporeal life support (ECLS). The increased bleeding tendency is partly due to an ECLS-induced thrombocytopenia and impaired platelet function. In the present study, we evaluated the effect of nitric oxide on the ECLS-induced platelet consumption and activation. Two identical in vitro ECLS circuits were primed with fresh, heparin-treated human blood and circulated for 24 hours. Nitric oxide (15, 40, or 77 ppm) was added to one of the oxygenators in each pair. Eight paired experiments were performed. Platelet count, plasma beta-thromboglobulin, platelet serotonin content, plasma nitrate, plasma cyclic guanosine monophosphate, and platelet membrane glycoprotein Ib were assayed before the start and at 0.5, 1, 3, 12, and 24 hours of perfusion. Plasma nitrate and plasma cyclic guanosine monophosphate levels were significantly higher in the nitric oxide circuits than in the control circuits (p < 0.01). Higher platelet counts (p < 0.01) and lower beta-thromboglobulin levels (p < 0.01) were observed in the nitric oxide circuits compared with the control circuits. However, no significant differences in platelet serotonin content or platelet membr...Continue Reading

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Citations

Feb 18, 1999·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·J Wassermann, C D Mazer
Jul 20, 2002·Current Opinion in Pediatrics·Ronald B Hirschl
Jul 9, 2003·Critical Care Medicine·Jens M KlingeJörg Dötsch
Mar 26, 1999·Journal of Biomaterials Science. Polymer Edition·J LiR C Eberhart
May 22, 1998·The Annals of Thoracic Surgery·K MellgrenH Wadenvik
Feb 4, 2011·Organogenesis·Melissa M Reynolds, Gail M Annich
Dec 12, 2012·The Journal of Thoracic and Cardiovascular Surgery·Paul A ChecchiaCharles B Huddleston
Apr 30, 1999·The Journal of Thoracic and Cardiovascular Surgery·Y SuzukiL H Edmunds
Oct 21, 2016·Intensive Care Medicine·Christopher JamesWarwick Butt
Dec 27, 2018·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Benjamin D CarrJonathan W Haft
Aug 29, 2003·Scandinavian Cardiovascular Journal : SCJ·M SkogbyK Mellgren
Dec 8, 2000·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·H T TevaearaiL K von Segesser
Aug 20, 2019·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·John M ToomasianRobert H Bartlett
Jul 25, 2019·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Avery C RossidisMarcus G Davey

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