Nitric oxide increases biofilm formation in Saccharomyces cerevisiae by activating the transcriptional factor Mac1p and thereby regulating the transmembrane protein Ctr1

Biotechnology for Biofuels
Leyun YangHanjie Ying

Abstract

Biofilms with immobilized cells encased in extracellular polymeric substance are beneficial for industrial fermentation. Their formation is regulated by various factors, including nitric oxide (NO), which is recognized as a quorum-sensing and signal molecule. The mechanisms by which NO regulates bacterial biofilms have been studied extensively and deeply, but were rarely studied in fungi. In this study, we observed the effects of low concentrations of NO on biofilm formation in Saccharomyces cerevisiae. Transcriptional and proteomic analyses were applied to study the mechanism of this regulation. Adding low concentrations of NO donors (SNP and NOC-18) enhanced biofilm formation of S. cerevisiae in immobilized carriers and plastics. Transcriptional and proteomic analyses revealed that expression levels of genes regulated by the transcription factor Mac1p was upregulated in biofilm cells under NO treatment. MAC1 promoted yeast biofilm formation which was independent of flocculation gene FLO11. Increased copper and iron contents, both of which were controlled by Mac1p in the NO-treated and MAC1-overexpressing cells, were not responsible for the increased biofilm formation. CTR1, one out of six genes regulated by MAC1, plays an imp...Continue Reading

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Citations

Jun 25, 2020·Proceedings of the National Academy of Sciences of the United States of America·Giuseppe IaniriJoseph Heitman
Apr 15, 2021·Applied Microbiology and Biotechnology·Deli ZhangYong Chen

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Datasets Mentioned

BETA
SRP153792
PXD010751

Methods Mentioned

BETA
Illumina sequencing
PCR
scanning electron microscopy
two-hybrid

Software Mentioned

[UNK]

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