Nitric oxide-induced resistance to lethal photooxidative damage in a breast tumor cell line

Free Radical Biology & Medicine
Magdalena NiziolekAlbert W Girotti

Abstract

The long-term effects of nitric oxide (NO) on cell susceptibility to photodynamic killing have been studied, using a human breast tumor line (COH-BR1). Subconfluent cells were exposed to a nonlethal dose of spermine NONOate (SPNO, 0.2 mM) and 20 h later were metabolically sensitized with protoporphyrin IX (PpIX) by incubating with 5-aminolevulinic acid. PpIX overproduced in mitochondria was allowed to diffuse to peripheral sites, including plasma membrane, after which a photooxidative challenge was imposed. Active (but not decomposed) SPNO made cells substantially more resistant to necrotic photokilling than non-SPNO-treated controls. A similar response to a tert-butyl hydroperoxide challenge was observed. Hyperresistance was detected approximately 8 h post-SPNO, maximized after approximately 20 h, and reflected diminished oxidant accumulation, as determined with 2',7'-dichlorofluorescein. Intracellular free iron determined with the fluorescent probe calcein rose to approximately 160% of the control level 6 h after SPNO, but declined to approximately 70% after 24 h. Immunoblot analyses revealed a rapid early (approximately 2 h post-NO) increase in heme oxygenase-1 level, followed by a gradual (4-20 h post-NO) increase in ferrit...Continue Reading

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Citations

Jan 30, 2013·Nitric Oxide : Biology and Chemistry·Valentina RapozziLuigi Emilio Xodo
Jan 10, 2013·Nitric Oxide : Biology and Chemistry·Vera KovalevaAnatoly Uzdensky
Nov 3, 2009·Nitric Oxide : Biology and Chemistry·Christoph V SuschekErnst E van Faassen
Apr 22, 2009·Journal of Photochemistry and Photobiology. B, Biology·Kimberley J ReevesNicola J Brown
Feb 13, 2009·Bioorganic & Medicinal Chemistry·Marzia B GariboldiElena Monti
Jan 1, 2014·Journal of Cellular and Molecular Medicine·Chunxia WangFeifan Guo
Jan 2, 2007·Biochemical and Biophysical Research Communications·Fuki YamamotoShigeru Taketani
Feb 24, 2015·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Albert W Girotti
Jan 25, 2013·International Journal of Molecular Sciences·Christian Opländer, Christoph V Suschek
Dec 11, 2014·World Journal of Clinical Oncology·María Julia LambertiViviana Alicia Rivarola
Oct 27, 2016·Journal of Biomedical Optics·Vera D Kovaleva, Anatoly B Uzdensky
Dec 15, 2019·Archives of Physiology and Biochemistry·Rachid AkkiAngela Marino
Sep 24, 2019·Photochemistry and Photobiology·Albert W Girotti
Aug 31, 2013·Israel Journal of Chemistry·David Kessel, John Reiners
Apr 19, 2020·Cell Biochemistry and Biophysics·Albert W Girotti, Witold Korytowski
Sep 2, 2019·Journal of Photochemistry and Photobiology. B, Biology·Stanislav V RodkinAnatoly B Uzdensky
Dec 15, 2019·Biochemical Pharmacology·Albert W Girotti, Jonathan M Fahey

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