Nitric oxide induces an increased Na+ conductance in identified neurons of Aplysia

Brain Research
M SawadaN Hara

Abstract

The ionic mechanism of the effects of micropressure ejections of hydroxylamine (HOA) and sodium nitroprusside (SNP), nitric oxide (NO) generators, on the membrane of identified neurons (R9-R12) of Aplysia kurodai was investigated with conventional voltage-clamp, micropressure ejection, and ion-substitution techniques. Micropressure ejection of HOA and SNP onto the neurons caused a marked depolarization in the unclamped neurons. Clamping the same neurons at their resting potential level (-60 mV) and reejecting HOA and SNP with the same dose produced a slow inward current (Ii(HOA) and Ii(SNP), 3-7 nA in amplitude, 15-60 s in duration) associated with an increase in input membrane conductance. Bath-applied hemoglobin (50 microM), a nitric oxide scavenger, almost completely blocked Ii(HOA) and Ii(SNP), and 3-isobutyl-1-methylxanthine (IBMX, 50 microM) prolonged and enhanced both Ii(HOA) and Ii(SNP). An intracellular injection of cyclic guanosine 3',5'-monophosphate (cGMP) into the same neurons produced a slow inward current (Ii(cGMP)) which resembled the responses to HOA and SNP, and this current was enhanced in IBMX. Bath-applied methylene blue (10 microM), an inhibitor of guanylate cyclase, significantly reduced Ii(HOA) and Ii(SN...Continue Reading

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Citations

Nov 19, 1997·Journal of Neuroscience Research·M SawadaG B Stefano
Jun 1, 1997·Invertebrate Neuroscience : in·J W Jacklet
Jan 15, 1999·Molecular Neurobiology·M Colasanti, G Venturini
May 17, 2005·Journal of the Neurological Sciences·David A Bechtold, Kenneth J Smith
Jul 10, 2003·Lancet Neurology·Kenneth J Smith, Hans Lassmann
Dec 1, 2007·The Journal of Physiology·Maria Vittoria PoddaClaudio Grassi
Jun 17, 2014·Neuroscience and Biobehavioral Reviews·Mehdi Ghasemi, Ali Fatemi
May 1, 1996·Journal of Neurobiology·A GelperinI R Cooke
Jul 2, 2002·Journal of Neurophysiology·Verónica AbudaraFrancisco R Morales
May 1, 1996·Journal of Neurobiology·M C Broillet, S Firestein

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