Nitric oxide induces metallothionein (MT) gene expression apparently by displacing zinc bound to MT

Toxicology Letters
K KatakaiMichael P Waalkes

Abstract

The metal binding protein metallothionein (MT) is involved in zinc homeostasis since it typically binds large amounts of zinc. Free zinc can control MT gene expression by interacting with metal-sensitive transcription factors. However, the precise factors governing intracellular release of metal ions from MT remain unknown. Aerobic nitric oxide (NO) can nitrosate thiol groups in proteins, and MT-bound cadmium is released by NO exposure. Thus, we hypothesized that NO may also be effective at displacing zinc from MT in cultured cells and that this could be an important physiological control mechanism in zinc homeostasis and utilization. In this study, DETA/NO, an agent that spontaneously generates NO with a 20-h half life in physiological media, was used to study the release of zinc from MT and the induction of MT in TRL1215 cells (a normal rat liver cell line). Zinc or cadmium was given at levels inducing MT production, followed by DETA/NO (20-200 microM) to produce controlled NO exposure in both cell lines. Although both metals activated MT gene expression, MT-I mRNA and MT protein were further increased when DETA/NO was given after zinc or cadmium treatment. Additionally, NO from DETA/NO clearly displaced MT-bound zinc, as evi...Continue Reading

References

May 11, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J LiuC D Klaassen
Jan 1, 1988·Toxicology and Applied Pharmacology·L D Lehman-McKeemanC D Klaassen
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·M A SchwarzJ R Lancaster
Oct 29, 1993·Biochemical and Biophysical Research Communications·M C KennedyD H Petering
Mar 17, 1997·FEBS Letters·D BerendjiK D Kröncke
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·W Maret, B L Vallee
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·L L PearceE S Levitan
Jan 8, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K D Kröncke, C Carlberg

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Citations

Mar 17, 2004·Pflügers Archiv : European journal of physiology·Sumon PalElias Aizenman
Mar 3, 2005·Nitric Oxide : Biology and Chemistry·Wei QuMichael P Waalkes
Mar 6, 2009·Plant Physiology·Roberto De MicheleFiorella Lo Schiavo
Jul 11, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Tridib ChakrabortyMalay Chatterjee
Dec 25, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Hui WangHongzhe Sun
Mar 3, 2004·Experimental Animals·Masaya KawaseKiyohito Yagi
Sep 7, 2011·Plant Science : an International Journal of Experimental Plant Biology·Magdalena Arasimowicz-JelonekEdward A Gwóźdź
Dec 17, 2008·The New Phytologist·Natalia Correa-AragundeLorenzo Lamattina
Mar 15, 2006·Mechanisms of Ageing and Development·Giovanni MusciMarco Colasanti
Sep 3, 2013·Oxidative Medicine and Cellular Longevity·Victor Manuel Blanco-AlvarezBertha Alicia Leon-Chavez
Jan 2, 2008·Experimental Gerontology·Manuela CasadeiMarco Colasanti
Sep 30, 2004·Free Radical Biology & Medicine·Ariel H B PoliandriBeatriz H Duvilanski
Dec 6, 2016·Metallomics : Integrated Biometal Science·Mohammad R Karim, David H Petering
Mar 29, 2014·Memórias do Instituto Oswaldo Cruz·Martha Elba Gonzalez-MejiaRicardo Pérez-Fuentes
Apr 20, 2007·Archives of Biochemistry and Biophysics·Klaus-D Kröncke
Mar 4, 2010·Human & Experimental Toxicology·Eliana A MilerBeatriz H Duvilanski
Apr 6, 2007·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Norio Itoh, Tomoki Kimura
Oct 5, 2006·Experimental Biology and Medicine·Mi Ja ChungSung-Joon Lee
May 26, 2004·The Journal of Nutritional Biochemistry·Keith M EriksonMichael Aschner

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