Nitric oxide inhibits hypoxia-induced impairment of human RBC deformability through reducing the cross-linking of membrane protein band 3

Journal of Cellular Biochemistry
Yajin ZhaoHongliang Zhu

Abstract

Nitric oxide (NO) prevents the decline of RBC deformability under high altitude and other ischemic and hypoxic conditions, but the clear mechanisms remain unknown. Here, we have carried out a systematic study to find the mechanisms of NO-induced regulation of RBC deformability under hypoxia. NO levels, RBCs membrane elongation index (EI), membrane protein band 3 methemoglobin (MetHb) were determined during hypoxia (0 to 120 minutes). To validate the role of NO in regulating RBC deformability, tests were also performed with a NO donor (sodium nitroprusside) or a NO synthase inhibitor (l-nitro-arginine methylester) under 60 minutes hypoxia. Hypoxia for 45 minutes increased NO levels from 25.65 ± 1.95 to 35.26 ± 2.01 μmol/L, and there was a plateau after 60 minutes hypoxia. The EI did not change before 45 minutes hypoxia, but decreased from 0.567 ± 0.019 to 0.409 ± 0.042 (30 Pa) after 60 minutes hypoxia. The cross-linking of band 3 and phosphotyrosine increased after 45 minutes hypoxia. All can be alleviated by supplement NO and aggregated by inhibiting NOS. However, the MetHb was not present this trend. NO may prevent decreased of RBCs deformability through reducing the cross-linking of membrane band 3 under hypoxia; this helps m...Continue Reading

References

Jan 1, 1971·British Journal of Haematology·J Stocks, T L Dormandy
Sep 23, 1994·Cell·H H Schmidt, U Walter
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D A WinkJ B Mitchell
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L J McDonald, J Moss
Mar 30, 1993·European Journal of Pharmacology·R Korbut, R J Gryglewski
Dec 1, 1995·The American Journal of Physiology·M L EllsworthH H Dietrich
Dec 1, 1996·The Biochemical Journal·G MinettiA Brovelli
Sep 29, 2001·Nature Immunology·C Bogdan
May 23, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Michael C KohnChristopher J Portier
Oct 9, 2002·Blood Cells, Molecules & Diseases·J G G DobbeC A Grimbergen
Jan 11, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Melek Bor-KucukatayOguz K Baskurt
May 3, 2003·Trends in Molecular Medicine·Matthew W FosterJonathan S Stamler
Mar 17, 2004·Cardiovascular Research·Antonio Martínez-Ruiz, Santiago Lamas
Feb 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·M Estela CampanellaPhilip S Low
Feb 16, 2005·Annual Review of Physiology·David J Singel, Jonathan S Stamler
Feb 22, 2005·Molecular Aspects of Medicine·Richard Bruckdorfer
Dec 22, 2005·Blood·Petra KleinbongardMalte Kelm
Sep 22, 2006·Antioxidants & Redox Signaling·Junhui SunElizabeth Murphy
May 8, 2007·Respiratory Physiology & Neurobiology·Agoston DosekZsolt Radak
Feb 17, 2009·Medicinal Research Reviews·Ernst E van FaassenMark T Gladwin
Apr 14, 2009·Nitric Oxide : Biology and Chemistry·Mehmet UyukluOguz K Baskurt
Apr 15, 2009·Physiology·Mary L EllsworthRandy S Sprague
May 7, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stephen C RogersAllan Doctor
Oct 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ian A LewisPhilip S Low
Jan 26, 2012·PloS One·Sirada SrihirunNathawut Sibmooh
Feb 23, 2012·Scientific Reports·Denny Z LevettUNKNOWN Caudwell Extreme Everest Research Group
Oct 3, 2012·Antioxidants & Redox Signaling·Joseph M Rifkind, Enika Nagababu

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.