Nitric oxide inhibits larval settlement in Amphibalanus amphitrite cyprids by repressing muscle locomotion and molting

Proteomics
Gen ZhangPei-Yuan Qian

Abstract

Nitric oxide (NO) is a universal signaling molecule and plays a negative role in the metamorphosis of many biphasic organisms. Recently, the NO/cGMP (cyclic guanosine monophosphate) signaling pathway was reported to repress larval settlement in the barnacle Amphibalanus amphitrite. To understand the underlying molecular mechanism, we analyzed changes in the proteome of A. amphitrite cyprids in response to different concentrations of the NO donor sodium nitroprusside (SNP; 62.5, 250, and 1000 μM) using a label-free proteomics method. Compared with the control, the expression of 106 proteins differed in all three treatments. These differentially expressed proteins were assigned to 13 pathways based on KEGG pathway enrichment analysis. SNP treatment stimulated the expression of heat shock proteins and arginine kinase, which are functionally related to NO synthases, increased the expression levels of glutathione transferases for detoxification, and activated the iron-mediated fatty acid degradation pathway and the citrate cycle through ferritin. Moreover, NO repressed the level of myosins and cuticular proteins, which indicated that NO might inhibit larval settlement in A. amphitrite by modulating the process of muscle locomotion a...Continue Reading

References

Nov 30, 1992·Biochemical and Biophysical Research Communications·N E Rogers, L J Ignarro
Dec 1, 1990·Archives of Biochemistry and Biophysics·D W Reif, R D Simmons
Nov 1, 1982·The Journal of Cell Biology·D L Granger, A L Lehninger
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·K Pantopoulos, M W Hentze
Mar 1, 1993·British Journal of Pharmacology·J AssreuyS Moncada
May 15, 1998·Nature·G García-CardeñaW C Sessa
Jan 9, 1999·The Journal of Biological Chemistry·A T BenderY Osawa
Sep 6, 2000·Pharmacology·J D Hayes, R C Strange
May 15, 2001·The Journal of Experimental Zoology·C D BishopB P Brandhorst
Dec 26, 2001·The Biological Bulletin·C D Bishop, B P Brandhorst
Feb 14, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Juliann G KiangGeorge C Tsokos
Sep 18, 2002·Trends in Plant Science·Ron Mittler
Sep 3, 2003·Evolution & Development·Cory D Bishop, Bruce P Brandhorst
Jul 30, 2004·Biochimica Et Biophysica Acta·Nathaniel TraasethCecilia Giulivi
Oct 12, 2004·Journal of Proteome Research·Lewis Y GeerStephen H Bryant
May 27, 2006·Experimental Parasitology·Mariana R MirandaClaudio A Pereira
Oct 12, 2007·The Biological Bulletin·Jan A PechenikMaia Leppo
Dec 17, 2009·Methods in Molecular Biology·Patrick G A Pedrioli
Oct 1, 2013·Neuroscience Letters·Lorenzo GallusMaria Angela Masini
Feb 1, 1999·The Biological Bulletin·S J Froggett, E M Leise

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.