Nitric oxide inhibits T cell adhesion and migration by down-regulation of beta1-integrin expression in immunologically liver-injured mice

International Immunopharmacology
Yang SunQiang Xu

Abstract

Our previous study has reported that nitric oxide (NO) exerts a protective role in immunologically liver-injured mice induced by delayed-type hypersensitivity to picryl chloride. To explore the mechanism of the protection, we have now examined the effect of NO on T cell adhesion and migration. First, we isolated hepatocytes and nonparenchymal cells from the liver-injured mice and separated the nonparenchymal cells into Kupffer cell-enriched and lymphocyte-enriched populations. When these hepatocytes or the fractions of nonparenchymal cells were co-cultured with spleen T cells of the liver-injured mice in a Transwell system, the adhesive potential of the T cells was significantly inhibited in the presence of hepatocytes or the Kupffer cell-enriched population but not the lymphocyte-enriched population of nonparenchymal cells. This effect was dependent on NO production. The NO synthase inhibitor N(G)-monomethyl-L-arginine (L-NMMA) could reverse this inhibition of cell adhesion and also decrease NO production. To confirm this effect of NO on T cells, we further examined the role of exogenous or endogenous NO on the adhesive activity of the Jurkat T cell line. As a result, the NO donor, S-nitroso-N-acetyl penicillamine (SNAP) cause...Continue Reading

References

Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Feb 1, 1996·The Journal of Surgical Research·D J WirthlinK S Ephgrave
Jun 14, 2000·Journal of Hepatology·C E CarnovaleM C Carrillo
Dec 29, 2000·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·J CaoA Koda
Aug 23, 2001·International Immunopharmacology·J W Coleman
Feb 16, 2002·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Q XuX M Zhang
May 16, 2002·Molecular Immunology·Vladimír HolánJana Pindjáková
Jun 18, 2002·Infection and Immunity·Claudia Farias BenjamimFernando Queiroz Cunha
Aug 29, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Gautam P YagnikDavid A Geller
Jan 21, 2004·Nitric Oxide : Biology and Chemistry·Daniela Dal SeccoFernando de Queiroz Cunha
Feb 14, 2004·Cardiovascular Research·Rainer SchulzGerd Heusch
Feb 21, 2004·International Immunopharmacology·Gaku MitsuiMasatoshi Kato
May 12, 2004·Cytokine·Juliano L FernandesMaria Heloisa S L Blotta

❮ Previous
Next ❯

Citations

Mar 12, 2013·Antioxidants & Redox Signaling·Djordje Miljković, Ivan Spasojević
Apr 2, 2011·Rejuvenation Research·Anthony OttoKetan Patel
Aug 27, 2013·Cell Biochemistry and Biophysics·Wen LiChunqing Zhang
Aug 1, 2014·Cancer Letters·Huiwen ChengLi Zuo
Mar 18, 2010·Immunopharmacology and Immunotoxicology·G MosayebiAbbas Mirshafiey

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Autoimmune Hepatitis

Autoimmune hepatitis formerly called lupoid hepatitis, is a chronic, autoimmune disease of the liver that occurs when the body's immune system attacks liver cells causing the liver to be inflamed. Discover the latest research on autoimmune hepatitis here.