PMID: 9164871May 15, 1997Paper

Nitric oxide inhibits the synthesis of type-II collagen without altering Col2A1 mRNA abundance: prolyl hydroxylase as a possible target

The Biochemical Journal
M CaoC H Evans

Abstract

The addition of human recombinant interleukin-1beta (IL-1beta) to cultures of lapine articular chondrocytes provoked the synthesis of large amounts of NO and reduced the production of type-II collagen. NG-Monomethyl-l-arginine (L-NMA), an inhibitor of NO synthase, strongly suppressed the production of NO and partially relieved the inhibition of collagen synthesis in response to IL-1beta. The NO donor S-nitrosoacetylpenicillamine (SNAP), on the other hand, inhibited collagen production. IL-1 lowered the abundance of Col2A1 mRNA in an NO-independent manner. Collectively, these data indicate that IL-1 suppresses collagen synthesis at two levels: a pretranslational level which is NO-independent, and a translational or post-translational level which is NO-mediated. These effects are presumably specific as L-NMA and SNAP had no effect on total protein synthesis or on the distribution of newly synthesized proteins between the cellular and extracellular compartments. Prolyl hydroxylase is an important enzyme in the post-translational processing of collagen, and its regulation and cofactor requirements suggest possible sensitivity to NO. Extracts of cells treated with IL-1 or SNAP had lower prolyl hydroxylase activity, and L-NMA was par...Continue Reading

Citations

May 18, 2001·FEMS Microbiology Letters·S StepanovićM Svabić-Vlahović
Nov 28, 2002·International Journal of Impotence Research·T J BivalacquaW J G Hellstrom
Jan 22, 2005·Annals of the New York Academy of Sciences·Gundula Schulze-TanzilMehdi Shakibaei
Jan 16, 2010·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Hao PengBo Qiu
Jun 30, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Era JainSilviya P Zustiak
Mar 12, 2020·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·T C LuchiA S Leopoldo
Aug 24, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·B FermorF Guilak
Sep 26, 2000·American Journal of Physiology. Cell Physiology·R K StuderC H Evans
Mar 15, 2003·Scandinavian Journal of Rheumatology·Katriina VuolteenahoEeve Moilanen
Aug 23, 2005·American Journal of Veterinary Research·Keiichi KurokiJames L Cook
Sep 14, 2007·Scandinavian Journal of Rheumatology·K VuolteenahoE Moilanen
Apr 14, 2005·Journal of the American Veterinary Medical Association·Kirsten M NeilMichael W Orth
Feb 21, 2006·The Journal of Biological Chemistry·Li ChenXing Li Wang
Jan 27, 2006·Rheumatology International·Kenta MatsudaTakashi Matsushita
Aug 23, 2011·The American Journal of Sports Medicine·Gerben M van BuulGerjo J V M van Osch
Dec 14, 2011·BMC Musculoskeletal Disorders·Martin H StradnerWinfried B Graninger
May 15, 2013·Arthritis and Rheumatism·Martin H StradnerWinfried B Graninger
Jul 12, 2011·Cellular and Molecular Life Sciences : CMLS·Urszula OleksiewiczGeorge Xinarianos
Jan 1, 2012·Scientifica·Paul R Gardner
Dec 21, 2000·Current Rheumatology Reports·A R AminS B Abramson
Dec 20, 2005·Rheumatology International·Wolfgang LorenzChristoph Trautmann
Nov 28, 2002·International Journal of Impotence Research·N F Gonzalez-CadavidJ Rajfer
Oct 14, 2004·Clinical Orthopaedics and Related Research·Mary B Goldring, Francis Berenbaum

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.